| 青花菜褐茎病的症状及其病原鉴定 |
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| 引用本文： | 王汉荣,王连平,方丽,李伟龙,苏英京,赵建阳.青花菜褐茎病的症状及其病原鉴定[J].浙江农业学报,2010,22(5):660. |
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| 作者姓名： | 王汉荣 王连平 方丽 李伟龙 苏英京 赵建阳 |
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| 作者单位： | 1. 浙江大学 农业与生物技术学院,浙江 杭州 310020；;2.浙江省农业科学院 植物保护与微生物研究所, 浙江 杭州 310021； ;3.浙江省临海市人民政府蔬菜办公室, 浙江 临海 314000； ;4.浙江省农作物管理局,浙江 杭州 310009 |
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| 基金项目： | 浙江省重大科技攻关项目，浙江省三农五方项目 |
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| 摘 要： | 青花菜褐茎病是近年来浙江省青花菜产区发生的一种新病害。对全省青花菜褐茎病的症状进行调查研究,对典型症状样本的病原菌进行了分离培养、镜检,发现青花菜褐茎病的病原菌不可分离培养,为专性寄生菌；病样镜检后发现病原菌孢囊梗呈二叉状分枝，小枝末端尖细，孢子囊椭圆形或卵形，大小为（24~27） μm×(15~20) μm，卵孢子球形，黄褐色，厚壁，直径30~40 μm；用青花菜的褐色发病组织对健康青花菜花球和植株进行人工接种能引起与田间青花菜褐茎病相同的症状；PCR扩增该病原菌的rDNA ITS序列并进行序列比对分析,发现该菌与寄生霜霉Hyaloperonospora parasitica相似度达95%以上,结合组织培养获得的病原形态特征,明确青花菜褐茎病病原为寄生霜霉(H. parasitica)。
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| 关 键 词： | 青花菜 褐茎病 寄生霜霉 |
Symptom of broccoli brown stem disease and identification of its pathogen |
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| WANG Han-rong,WANG Lian-ping,Fang Li,LI Wei-long,SU Ying-jing,Zhao Jian-yang.Symptom of broccoli brown stem disease and identification of its pathogen[J].Acta Agriculturae Zhejiangensis,2010,22(5):660. |
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| Authors: | WANG Han-rong WANG Lian-ping Fang Li LI Wei-long SU Ying-jing Zhao Jian-yang |
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| Institution: | 1College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310020, China; 2 Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China；3 Vegetable Office in People Government of Linhai, Zhejiang Province, Linhai 314000, China; 4Bureau of Agricultural Management of Zhejiang Province, Hangzhou 310009, China |
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| Abstract: | Broccoli brown stem, a new disease, was widespread in Zhejiang Province in recent years. To understand and control the disease, lots of model symptom samples were collected to separate and culture. The results indicated that the pathogen, as an obligate parasite, depended on the host plants and could not be cultured in vitro. In microscope, the sporangiophores of pathogen tapered on the tips with a shape like letter “Y”, the sporangiums of which were oval or eggs, (24-27) μｍ×(15-20) μｍ, then the oospores with thick wall were roundness with snuff color. When inoculated some mycelium from sick tissues on the surface, the symptoms same as in fields appeared on healthy broccoli leaves and stems. On the other hand, rDNA ITS (Internal Transcribed Spacers) of pathogens were cloned and sequenced, and its similarity to Hyaloperonospora parasitica was more than 95%. Combining the molecular results with the morphological characters, it can be definitely certain that broccoli brown stem was caused by Hyaloperonospora parasitic. |
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| Keywords: | Brassica oleracea brown stem disease Hyaloperonospora parasitica |
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