Structural and Immunological Activity Characterization of a Polysaccharide Isolated from Meretrix meretrix Linnaeus |
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| Authors: | Li Li Heng Li Jianying Qian Yongfeng He Jialin Zheng Zhenming Lu Zhenghong Xu Jinsong Shi |
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| Institution: | School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China; (L.L.); (H.L.); (J.Q.); (Y.H.); (J.Z.); (Z.L.); (Z.X.) |
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| Abstract: | Polysaccharides from marine clams perform various biological activities, whereas information on structure is scarce. Here, a water-soluble polysaccharide MMPX-B2 was isolated from Meretrix meretrix Linnaeus. The proposed structure was deduced through characterization and its immunological activity was investigated. MMPX-B2 consisted of d-glucose and d-galctose residues at a molar ratio of 3.51:1.00. The average molecular weight of MMPX-B2 was 510 kDa. This polysaccharide possessed a main chain of (1→4)-linked-α-d-glucopyranosyl residues, partially substituted at the C-6 position by a few terminal β-d-galactose residues or branched chains consisting of (1→3)-linked β-d-galactose residues. Preliminary immunological tests in vitro showed that MMPX-B2 could stimulate the murine macrophages to release various cytokines, and the structure-activity relationship was then established. The present study demonstrated the potential immunological activity of MMPX-B2, and provided references for studying the active ingredients in M. meretrix. |
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| Keywords: | Meretrix meretrix Linnaeus polysaccharide structure immunological activity |
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