| 应用间接ELISA检测鸡痘病毒抗体方法的建立 |
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| 引用本文： | 张体银,李军伟,孙蕾,孙学辉,张如宽,刘秀梵.应用间接ELISA检测鸡痘病毒抗体方法的建立[J].中国兽医学报,2005,25(2):128-130. |
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| 作者姓名： | 张体银 李军伟 孙蕾 孙学辉 张如宽 刘秀梵 |
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| 作者单位： | 扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009 |
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| 基金项目： | 国家“863”计划项目(2001AA213041)，科技部农业科技成果转化资金项目(OIEFN213201078) |
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| 摘 要： | 建立了检测鸡痘病毒抗体的间接ELISA方法。应用该方法检测鸡痘阳性血清，其灵敏度为琼脂扩散试验的400～800倍，而且还具有特异性强、操作简便、快速等特点。
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| 关 键 词： | 间接ELISA 鸡痘病毒 抗体检测 鸡痘 |
| 文章编号： | 1005-4545(2005)02-0128-03 |
| 修稿时间： | 2003年11月19 |
Development of Indirect ELISA for Detection of Antibodies to Fowlpox Virus |
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| ZHANG Ti-yin,LI Jun-wei,SUN Lei,SUN Xue-Hui,ZHANG Ru-kuan,LIU Xiu-fan.Development of Indirect ELISA for Detection of Antibodies to Fowlpox Virus[J].Chinese Journal of Veterinary Science,2005,25(2):128-130. |
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| Authors: | ZHANG Ti-yin LI Jun-wei SUN Lei SUN Xue-Hui ZHANG Ru-kuan LIU Xiu-fan |
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| Abstract: | An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to fowlpox virus was developed and compared with agar gel diffusion technique. These assays were used to examine the sera from chickens vaccinated experimentally, and the results revealed that the ELISA was 400-800 times more sensitive than AGID. It was simple, convenient, specific, sensitive and rapid for detecting of antibody to fowlpox virus. |
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| Keywords: | ELISA fowlpox virus antibody |
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