| 影响白菜游离小孢子培养关键因素分析 |
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| 引用本文： | 耿建峰,侯喜林,张晓伟,蒋武生,原玉香,韩永平,姚秋菊,成妍,李英.影响白菜游离小孢子培养关键因素分析[J].园艺学报,2007,34(1):111-116. |
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| 作者姓名： | 耿建峰 侯喜林 张晓伟 蒋武生 原玉香 韩永平 姚秋菊 成妍 李英 |
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| 作者单位： | ( 南京农业大学作物遗传与种质创新国家重点实验室, 南京210095; 河南省农业科学院园艺研究所, 郑州450002) |
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| 基金项目： | 教育部科学技术研究重点项目;国家高技术研究发展计划(863计划);国家重点实验室基金;江苏省高技术研究发展计划项目 |
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| 摘 要： | 对影响白菜游离小孢子培养的关键因素进行分析研究。单因素研究结果表明: 54个基因型之间的小孢子胚诱导率差异显著; 对供体材料低温处理, 在0～5 d范围内小孢子胚诱导率差异不大, 超过5 d则明显降低; 高温诱导在12～60 h范围内差异不大, 超过此范围则小孢子胚诱导率明显降低; NLN培养基中添加生长素(NAA) 和细胞分裂素(6-BA) 对小孢子胚诱导率影响不大, 添加的浓度过大时小孢子胚诱导率反而降低; 活性炭的有无和浓度大小对小孢子胚诱导率影响极大。通过基因型、生长素、细胞分裂素、活性炭4因素分析表明: 基因型之间差异显著, 活性炭不同浓度之间差异显著; 基因型与活性炭不同浓度之间的互作差异显著; 其它的5个一级互作、4个二级互作和1个三级互作差异均不显著。
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| 关 键 词： | 白菜 小孢子培养 |
| 文章编号： | 0513-353X（2007）01-0111-06 |
| 收稿时间： | 2006-04-13 |
| 修稿时间： | 2006-04-132006-07-28 |
Isolated Microspore Culture in Brassica campestris ssp. chinensis |
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| GENG Jian-feng,HOU Xi-lin,ZHANG Xiao-wei,JIANG Wu-sheng,YUAN Yu-xiang,HAN Yong-ping,YAO Qiu-ju,CHENG Yan,LI Ying.Isolated Microspore Culture in Brassica campestris ssp. chinensis[J].Acta Horticulturae Sinica,2007,34(1):111-116. |
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| Authors: | GENG Jian-feng HOU Xi-lin ZHANG Xiao-wei JIANG Wu-sheng YUAN Yu-xiang HAN Yong-ping YAO Qiu-ju CHENG Yan LI Ying |
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| Institution: | (;1. National Key Laboratory of Crop Genetic and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;2. Institute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou 450002,China) |
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| Abstract: | Several factors affecting isolated microspore culture of non-heading Chinese cabbage(Brassica campestris ssp.chinensis)were studied in experiments.The results from mono-factor experiments showed that there were significant differences in the frequency of microspore-induced embryos among different genotypes.In the low temperature treatment,no significant differences were found during 0-5 days,while the frequencies were obviously lower when the period was longer than five days.There were no significant differences in high temperature when the incubation time was within 12-60 h,but the frequencies of microspore-induced embryos were much lower over this duration.The addition of auxins(NAA)and the cytokinins(6-BA)in NLN medium showed no significant effect on the frequencies of microspore-induced embryos,but when the concentration was excessive,the frequencies decreased.The presence and concentration of activated charcoal had a great influence on the frequency of microspore-induced embryos.Moreover,four-factor analysis of genotype,auxin,cytokinins and activated charcoal concentrations indicated that there were significant differences among different genotypes and activated charcoal concentrations,respectively,and also significant differences among these two interactions,while other interactions remained non-significant. |
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| Keywords: | Brassica campestris ssp chinensis Microspore culture |
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