| 地衣芽孢杆菌16S-ITS标记及其特异性分析 |
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| 引用本文： | 陈冲,佟建明,张潞生.地衣芽孢杆菌16S-ITS标记及其特异性分析[J].农业科学与技术,2011(11):1572-1573,1588. |
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| 作者姓名： | 陈冲 佟建明 张潞生 |
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| 作者单位： | 山西省农业科学院果树研究所;中国农业科学院北京畜牧兽医研究所;中国农业大学农学与生物技术学院 |
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| 摘 要： | 目的]建立地衣芽孢杆菌的分子鉴定方法。方法]通过对地衣芽孢杆菌TS-01的16S和ITS序列进行克隆测序和差异性分析,以该区序列为靶序列设计地衣芽孢菌TS-01的特异性引物,并用该引物扩增全部受试菌种。结果]从ITS和16Sr DNA区间设计了地衣芽孢杆菌种特异性引物,特异性引物PCR的最佳退火温度为67.2℃,循环数为24个;地衣芽孢杆菌TS-01可扩增出1条905bp的标记片段,其他受试菌株均为阴性,从而证明试验得到了在种水平上对该菌种进行准确鉴定的特异16S-ITS标记。结论]地衣芽孢杆菌TS-01分子鉴定方法的建立,为地衣芽孢杆菌的分子诊断奠定了基础。
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| 关 键 词： | 地衣芽孢杆菌 特异引物 16S ITS |
Analysis on 16S-ITS Marker from Bacillus licheniformis and Its Specificity |
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| CHEN Chong,TONG Jian-ming,ZHANG Lu-sheng.Analysis on 16S-ITS Marker from Bacillus licheniformis and Its Specificity[J].Agricultural Science ＆ Technology,2011(11):1572-1573,1588. |
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| Authors: | CHEN Chong TONG Jian-ming ZHANG Lu-sheng |
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| Institution: | 1. Pomology Institute, Shanxi Academy of Agricultural Sciences, Taigu 030815; 2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094; 3. College of Agriculture and Biotechnology, China Agricultural University, Beijing 100094 |
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| Abstract: | Objective] This study aimed to establish molecular identification methods for Bacillus licheniformis. Method] Based on clone sequencing and difference analysis for 16S and ITS sequences of B. licheniformis TS-01, specific primers were designed using region sequences as the targets used for amplifying all test strains. Result] The specific primers of B. licheniformis were designed from the ITS and 16S rDNA regions. The optimal annealing temperature of the specific primers for PCR was 67.2 ℃ with 24 cycles. A 905 bp marker fragment was amplified for B. licheniformis TS-01, while all other test strains showed negative results. This indicated that a specific 16S-ITS marker was obtained, which accurately identified the strain at the species level. Conclusion] This molecular identification method for B. licheniformis TS-01 has laid the foundation for molecular diagnosis of B. licheniformis. |
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| Keywords: | Bacillus licheniformis Specific primer 16S ITS |
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