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Molecular approaches for enhancing sweetness in fruits and vegetables
Authors:Akula Nookaraju  Chandrama P Upadhyaya  Shashank K Pandey  Ko Eun Young  Se Jin Hong  Suk Keun Park  Se Won Park
Institution:1. Department of Molecular Biotechnology, Konkuk University, 1, Hwayang-dong, Gwangin-gu, Seoul 143 701, Republic of Korea;2. Department of Applied Plant Science, Gangneung-Wonju National University, Gangneung 210 702, Republic of Korea
Abstract:The quality of fruits and vegetables is mainly dependant on the sweetness determined by the level of soluble sugars such as glucose, fructose and sucrose. Other fruit quality parameters include Brix content, acidity, aroma, color, size and shape. Total sugar content in fruits and vegetables is a function of genetic, nutritional, environmental and developmental factors. Understanding the factors controlling sweetness is important to design strategies for enhancing quality of fruits and vegetables. Modifying the activity of enzymes in carbohydrate metabolism such as sucrose synthase (SuSy), acid invertase, ADP-glucose pyrophosphorylase (AGPase), sucrose phosphate synthase (SPS) and sucrose transporters were found to influence carbohydrate partitioning and sucrose accumulation in sink tissues of several food crops. Plant based taste-modifying sweet proteins such as brazzein, cucurmin, mabinlin, monellin, miraculin, neoculin and thaumatin have potential application for developing transgenic plants to improve the sweetness and quality of fruits and vegetables. The present review envisages various cultural, breeding and molecular approaches used for enhancing sugar content and sweetness in fruits and vegetables.
Keywords:AA  acetaldehyde  AGPase  ADP-glucose pyrophosphorylase  AI  acid invertase  EIN3  ethylene insensitive 3  FBPase  fructose-1  6-biphosphatase  FRK  fructokinase  GLK  glucokinase  HXK  hexokinase  Pdc  pyruvate decarboxylase  PFP  pyrophosphate d-frucrose-6-phosphate 1-phosphotransferase  PGM  phosphoglucomutase  SAMase  S-adenosyl methionine hydrolase  SI  sucrose isomerase  snRK1  SNF1-related protein kinase  SoSUT1  spinach sucrose transporter 1  SPS  sucrose phosphate synthase  1-SST  sucrose:sucrose-1-fructosyl transferase  SuP  sucrose phosphorylase  SuSy  sucrose synthase  SUT  sucrose transporter  S6PDH  sorbitol-6-phosphate dehydrogenase  SXD1  sucrose export defective 1  TSS  total soluble sugars  UDPase  uridine diphosphate-glucose pyrophosphorylase
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