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Intra-cultivar variability of three major olive cultivars grown in different areas of central-southern Italy and studied using microsatellite markers
Authors:Innocenzo Muzzalupo  Adriana Chiappetta  Cinzia Benincasa  Enzo Perri
Institution:1. Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di ricerca per l’olivicoltura e l’industria olearia (CRA-OLI), C.da Li Rocchi, 87036 Rende, (CS), Italy;2. Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di ricerca per le produzioni foraggere e lattiero-casearie (CRA-FLC), Via A. Lombardo, 26900 Lodi, Italy;3. Università degli Studi della Calabria, Dip. Ecologia, 87036 Arcavacata di Rende, (CS), Italy
Abstract:A collection of 70 olive samples, originating from diverse areas in central-southern Italy (Abruzzo, Apulia, Calabria, and Umbria) and corresponding to 3 major cultivars denominations (‘Carolea’, ‘Coratina’ and ‘Frantoio’), was genotyped at 10 microsatellite loci. In total, 44 alleles with a mean number of 4.4 alleles per locus were detected. The molecular analysis, allowed the study to show a clear genetic diversity between the three cultivars ‘Carolea’, ‘Coratina’ and ‘Frantoio’ and to state that ‘Carolea’ is a polyclonal cultivar, while ‘Coratina’ and ‘Frantoio’, are probably monoclonal ones. The analysis of intra-varietal polymorphism, through the SSR analysis, proved to be very useful both for varietal identification and for intra-varietal ones. Our work shows that the current designations of olive cultivars fall short of describing the genetic variability among economically important plant material. A thorough investigation of the existing variability will prove of major importance for both management and economic production of olive trees.
Keywords:Olea europaea  Fingerprinting  Genetic identity  Molecular markers  Intra-cultivar polymorphism  Cultivar families
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