High frequency plant regeneration through adventitious multiple shoot organogenesis in epicotyl explants of Indian gooseberry (Emblica officinalis Gaertn) |
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| Authors: | P Nayak PR Behera M Thirunavoukkarasu PK Chand |
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| Institution: | 1. Natural Products Department, Institute of Minerals and Materials Technology (CSIR), Bhubaneswar 751013, India;2. Post Graduate Department of Botany, Utkal University, Bhubaneswar 751 005, India |
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| Abstract: | Shoots regenerated adventitiously on epicotyl segments from in vitro seedlings of Emblica officinalis var. ‘Kanchan’. Epicotyls derived from 2-week-old aseptic seedlings were most responsive and produced a maximum number of 303 shoots per explant in Murashige and Skoog (1962) medium (MS) augmented with 8.8 μM N6-benzyladenine (BA) + 1.425 μM indole-3-acetic acid (IAA). Shoots readily elongated in MS lacking growth regulators and rooted in half-salt-strength MS (1/2 MS) supplemented with indole-3-butyric acid (IBA) or α-naphthalene acetic acid (NAA). The highest rooting response was recorded in 1/2 MS containing 14.7 μM IBA. Plantlets were acclimatized inside the green house and 80% of the plantlets survived on transfer to garden soil. |
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| Keywords: | Emblica officinalis Euphorbiaceae Epicotyl Adventitious regeneration |
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