Induction of disease resistance and ROS metabolism in navel oranges by chitosan |
| |
| Authors: | Kaifang Zeng Yuyan Deng Jian Ming Lili Deng |
| |
| Institution: | 1. College of Food Science, Southwest University, Chongqing 400715, PR China;2. Department of Food Science, Cornell University, Stocking Hall, Ithaca, NY 14853, USA |
| |
| Abstract: | The objective of this work was to evaluate how disease resistance and reactive oxygen species (ROS) metabolism in harvested navel oranges (Citrus sinensis L. Osbeck) may be affected by chitosan. Fresh navel oranges were treated with 2% chitosan or 0.5% glacial acetic acid (control) solution for 1 min, and some were inoculated with Penicillium italicum and Penicillium digitatum. Then, the fruit were stored at 20 °C and 85–95% RH. Treatment with 2% chitosan significantly reduced the disease incidence and the lesion diameter compared with control fruit. This treatment effectively enhanced the activities of peroxidase (POD) and superoxide dismutase (SOD), and levels of glutathione (GSH) and hydrogen peroxide (H2O2), inhibited the activities of catalase (CAT) and the decreases of ascorbate (AsA) content during navel orange fruits storage. Ascorbate peroxidase (APX) activity in the navel orange fruit was induced slightly by the chitosan treatment during 14–21 days storage. However, glutathione reductase (GR) activity in the fruit was not enhanced by the chitosan treatment. These results indicated that chitosan treatment could induce the navel orange fruit disease resistance by regulating the H2O2 levels, antioxidant enzyme and ascorbate–glutathione cycle. |
| |
| Keywords: | ROS reactive oxygen species APX ascorbate peroxidase CAT catalase GR glutathione reductase AsA ascorbate H2O2 hydrogen peroxide POD peroxidase SOD superoxide dismutase GSH glutathione |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
