Evaluation of genetic diversity among some Iranian wild asparagus populations using morphological characteristics and RAPD markers |
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| Authors: | Behrooz Sarabi Mohammad Reza Hassandokht Mohammad Esmail Hassani Taimour Ramak Masoumi Tim Rich |
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| Institution: | 1. Department of Horticultural Sciences, Faculty of Agriculture, University of Tehran, Karaj, Iran;2. Faculty of Agriculture, Food and Natural Resources, The University of Sydney, Australia;3. Department of Biodiversity & Systematic Biology, Welsh National Herbarium, National Museum Wales, UK |
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| Abstract: | An evaluation of genetic diversity in 39 wild asparagus populations was carried out using morphological and RAPD markers. A combination of morphological traits and random RAPD primers was used to examine the level of genetic variation and polymorphisms among the populations. A factor analysis using Ward's method on mean values of morphological characteristics indicated seven main factors resulting in four groups. Analysis of polymorphic bands using Jaccard's similarity coefficient indicated that genetic similarity ranged between 0.71 and 0.29. At a similarity level of 0.64, the populations were divided in three sub-clusters, containing 34, four and one populations, respectively. Significant regression associations were found between 21 morphological characteristics and 18 RAPD markers, revealing some informative markers associated with some traits. The highest R2 was related to 18 RAPD markers associated with gender (53.5%) that among them BA-042000 had a maximum R2. The results showed that Iranian wild asparagus with its high levels of genetic variation could be considered as a valuable gene pool for future asparagus breeding programs. Furthermore, it could be inferred that morphological characteristics and RAPD markers are suitable tools to discriminate asparagus populations for the evaluation of genetic diversity. |
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| Keywords: | Asparagus Factor analysis Gene pool Polymorphic bands Regression association |
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