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甘蔗品种“粤糖55号”的离体快速繁殖
引用本文:何海旺,许春燕,李文,李创珍,韦善清,何龙飞.甘蔗品种“粤糖55号”的离体快速繁殖[J].中国甜菜,2012(2):63-65.
作者姓名:何海旺  许春燕  李文  李创珍  韦善清  何龙飞
作者单位:[1]广西大学农学院,南宁530005 [2]广西农业科学院经济作物研究所,南宁530007
基金项目:2009年广西农业综合开发项目.
摘    要:以甘蔗品种"粤糖55号"为材料,对其离体快繁技术进行了研究。结果表明,用带腋芽的甘蔗茎段作为外植体,经0.1%HgCl2消毒7min后,接种到MS+6-BA 1.0 mg/L+NAA 0.2 mg/L的培养基上进行启动培养,能够诱导丛生芽。丛生芽继代和生根最优的培养基分别为MS+6-BA 1.0 mg/L和MS+NAA 2.0mg/L。甘蔗组培苗生根炼苗后,移到木糠中进行假植,成活率为90%~95%。

关 键 词:甘蔗  粤糖55号  腋芽  离体快繁

Rapid Micropropagation of Sugarcane Variety YT55
Authors:HE Hai-wang  XU Chun-yan  LI Wen  LI Chuang-zhen  WEI Shan-qing  HE Long-fei
Institution:1. College of A gronarny, Guangxi University, Nanning 530004, China ; 2. Industrial Crop Research Institute, Guangxi Academy of Agricultural Sciences ,Nanning 530007, China)
Abstract:The rapid micropropagation technique of sugarcane variety YT55 was studied. Lateral buds as explants source were disinfected by 0.1% solution of mercuric chloride for 7 minutes,then to plant in MS medium with 1.0 mg/L 6-BA and 0.2 mg/L NAA for shoot inducing. The results showed that lots of plantlets were induced. MS was the optimum medium with 1.0 mg/L 6-BA and the medium with 2.0 mg/L NAA for rapid micropropagation and rooting response,respectively. The survival rate was between 90% and 95% when the sugarcane plantlets were transplanted in the medium of wood chaff.
Keywords:sugarcane  YT55  lateral buds  rapid micropropagation
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