甘蔗品种“粤糖55号”的离体快速繁殖 |
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引用本文: | 何海旺,许春燕,李文,李创珍,韦善清,何龙飞.甘蔗品种“粤糖55号”的离体快速繁殖[J].中国甜菜,2012(2):63-65. |
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作者姓名: | 何海旺 许春燕 李文 李创珍 韦善清 何龙飞 |
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作者单位: | [1]广西大学农学院,南宁530005 [2]广西农业科学院经济作物研究所,南宁530007 |
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基金项目: | 2009年广西农业综合开发项目. |
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摘 要: | 以甘蔗品种"粤糖55号"为材料,对其离体快繁技术进行了研究。结果表明,用带腋芽的甘蔗茎段作为外植体,经0.1%HgCl2消毒7min后,接种到MS+6-BA 1.0 mg/L+NAA 0.2 mg/L的培养基上进行启动培养,能够诱导丛生芽。丛生芽继代和生根最优的培养基分别为MS+6-BA 1.0 mg/L和MS+NAA 2.0mg/L。甘蔗组培苗生根炼苗后,移到木糠中进行假植,成活率为90%~95%。
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关 键 词: | 甘蔗 粤糖55号 腋芽 离体快繁 |
Rapid Micropropagation of Sugarcane Variety YT55 |
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Authors: | HE Hai-wang XU Chun-yan LI Wen LI Chuang-zhen WEI Shan-qing HE Long-fei |
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Institution: | 1. College of A gronarny, Guangxi University, Nanning 530004, China ; 2. Industrial Crop Research Institute, Guangxi Academy of Agricultural Sciences ,Nanning 530007, China) |
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Abstract: | The rapid micropropagation technique of sugarcane variety YT55 was studied. Lateral buds as explants source were disinfected by 0.1% solution of mercuric chloride for 7 minutes,then to plant in MS medium with 1.0 mg/L 6-BA and 0.2 mg/L NAA for shoot inducing. The results showed that lots of plantlets were induced. MS was the optimum medium with 1.0 mg/L 6-BA and the medium with 2.0 mg/L NAA for rapid micropropagation and rooting response,respectively. The survival rate was between 90% and 95% when the sugarcane plantlets were transplanted in the medium of wood chaff. |
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Keywords: | sugarcane YT55 lateral buds rapid micropropagation |
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