| 甘露醇对马铃薯试管苗生长和有效保存期的影响 |
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| 引用本文： | 黄萍,马朝宏,颜谦.甘露醇对马铃薯试管苗生长和有效保存期的影响[J].西南农业学报,2011,24(6). |
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| 作者姓名： | 黄萍 马朝宏 颜谦 |
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| 作者单位： | 1. 贵州省生物技术研究所,贵州贵阳,550006
2. 贵州省农业科学院科技开发处,贵州贵阳,550006 |
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| 基金项目： | 贵州省重大专项“贵州省马铃薯产业化关键技术研究与示范之子项目1:贵州省马铃薯种质资源库建设”[黔科合重大专项字(2008)6009]; 贵州省创新平台建设项目“贵州薯类育种及产业化研发平台建设”[黔科合院所创能(2010)4006] |
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| 摘 要： | 为给马铃薯试管苗生长和有效保存提供试验依据,以费乌瑞它脱毒试管苗为试验材料,添加不同甘露醇浓度(1％～5％)的继代培养基对其进行增殖培养,比较不同甘露醇浓度对试管苗生长和有效保存期的影响.结果表明:所试甘露醇浓度均对马铃薯试管苗的生长有不同程度的抑制作用,同时还延长了试管苗的保存时间,其中以继代培养基中添加1％～2％的甘露醇的保存效果最为理想,可使试管苗保存7个月以上,成活率达50％,且不影响试管苗继代.
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| 关 键 词： | 马铃薯 脱毒试管苗 甘露醇 继代 |
Effect of Mannital on Growth and Preservation of Virus-free Potato Plantlets |
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| HUANG Ping
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MA Chao-hong
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YAN Qian.Effect of Mannital on Growth and Preservation of Virus-free Potato Plantlets[J].Southwest China Journal of Agricultural Sciences,2011,24(6). |
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| Authors: | HUANG Ping MA Chao-hong YAN Qian |
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| Institution: | HUANG Ping1,MA Chao-hong2*,YAN Qian1(1.Guizhou Biotechnology Institute,Guizhou Guiyang 550006,2.Department of Science and Technology Development,Guizhou Academy of Agriculture Sciences,China) |
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| Abstract: | Taking virus-free plantlets of Favorite as the experimental materials and the multiplication culture medium supplemented with mannital of different concentrations(1 %-5 %) as the regard proliferation medium,the effect of mannital of different concentrations on the growth and preservation of tube plantlets was studied to provide a reference for the growth and preservation of tube plantlets.The results showed that not only did mannital inhibit the growth of the tube plantlets,but it prolonged the preservation... |
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| Keywords: | Potato In vitro virus-free plantlets Mannitol Multiplication culture |
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