Temperature and storage period over spermatic parameters of jundiá, Rhamdia quelen (Quoy & Gaimard, 1824) |
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| Authors: | Eduardo Antônio Sanches Giovano Neumann César Pereira Rebechi de Toledo Robie Allan Bombardelli Pitágoras Augusto Piana Elizabeth Romagosa |
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| Institution: | 1. Aquaculture Centre, State University of Sao Paulo, , Jaboticabal, S?o Paulo, Brazil;2. State University of West Paraná, , Toledo, Paraná, Brazil;3. Fishery Institute – APTA, , Sao Paulo, S?o Paulo, Brazil |
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| Abstract: | The aim of this work was to evaluate the spermatic parameters of jundia, Rhamdia quelen, semen stored for short periods under different temperatures. Fifteen males were used in a time factorial experimental design (13 × 3 × 3 × 3). The sperm was stored at: 15; 25 and 35°C and activated in water at: 15; 25 and 35°C each, respectively, within storage periods of: 0; 2; 4; 6; 8; 10; 12; 16; 20; 24; 32; 40 and 48 h after collection. The treatments were performed in triplicates and in sequential protocols every 50 h. The motility parameters were evaluated using Pearson's correlation analysis and the significant parameters (P < 0.05) were summarized using principal component analysis forming two predicted groups. Group 01 composed the following parameters: curvilinear velocity, wobble and linearity; group 02 comprised average path velocity, straight line velocity, beating cross frequency and motility rate. Effects (P < 0.05) of treatments were observed only in group 02, with interaction between time and temperature of exposure and between exposure time and water temperature. Superior results of spermatic parameters of group 02 were observed right after collection. However, the exposure and spermatic activation at 15°C assured significant results 48 h after collection when compared with 25 and 35°C. |
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| Keywords: | artificial reproduction computer assisted sperm analysis computerized analysis gamete conservation semen |
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