| 高温胁迫对刺参耐高温群体和普通群体主要免疫酶活力的影响 |
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| 引用本文： | 刘石林,茹小尚,徐勤增,柏雨岑,李静,张立斌,杨红生.高温胁迫对刺参耐高温群体和普通群体主要免疫酶活力的影响[J].中国水产科学,2016,23(2):344-351. |
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| 作者姓名： | 刘石林 茹小尚 徐勤增 柏雨岑 李静 张立斌 杨红生 |
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| 作者单位： | 1. 中国海洋大学水产学院，山东青岛 266003; 中国科学院海洋研究所，海洋生态与环境科学重点实验室，山东青岛 266071;2. 中国海洋大学水产学院,山东青岛,266003;3. 中国科学院海洋研究所，海洋生态与环境科学重点实验室，山东青岛 266071;4. 山东东方海洋科技股份有限公司,山东烟台,264003 |
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| 基金项目： | 国家863计划项目-高值海珍品良种培育(2012AA10A412)，山东省农业良种工程课题-速生抗病耐高温刺参良种选育 |
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| 摘 要： | 以定向选育的刺参(Apostichopus japonicus)耐高温品系子三代(F3组)和未经选育的普通刺参(C组)1龄幼参(13.6±1.8)g/头]为研究对象,在室内模拟池塘夏季高温环境,比较了两个群体在25℃、27℃、29℃、31℃、32℃和33℃6个温度梯度下体壁抗氧化酶(SOD、CAT和T-AOC)、水解酶(ACP和AKP)和溶菌酶(LSZ)活力的差异。结果表明,在抗氧化酶活力方面,F3和C组刺参SOD活力均随温度的升高呈先升高后降低的趋势,且当温度为31℃和32℃时F3组SOD活力显著高于C组(P0.05);F3组CAT活力在实验温度范围内均高于C组,且当温度为27~31℃和33℃时差异显著(P0.05);F3组T-AOC活力在各温度下均显著高于C组(P0.05)。在水解酶活力方面,F3组的ACP活力在各温度下均高于C组,且除31℃外,其余温度下均差异显著(P0.05);F3组AKP活力在25℃、27℃和31℃下显著高于C组(P0.05),其余温度下差异不显著。F3和C组LSZ活力均随水温的上升呈先升高后降低的趋势,且在27~31℃范围内F3组LSZ活力显著高于C组(P0.05)。结果表明,经过定向选育的耐高温品系F3对高温的耐受能力获得了提高,实验结果为耐高温刺参良种选育提供了理论依据。
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| 关 键 词： | 刺参 耐高温品系 抗氧化酶 水解酶 溶菌酶 |
| 修稿时间： | 2016/3/14 0:00:00 |
Effects of high-temperature stress on several immune enzyme activities of Apostichopus japonicus thermotolerant and normal species |
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| LIU Shilin,RU Xiaoshang,XU Qinzeng,BAI Yucen,LI Jing,ZHANG Libin,YANG Hongsheng.Effects of high-temperature stress on several immune enzyme activities of Apostichopus japonicus thermotolerant and normal species[J].Journal of Fishery Sciences of China,2016,23(2):344-351. |
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| Authors: | LIU Shilin RU Xiaoshang XU Qinzeng BAI Yucen LI Jing ZHANG Libin YANG Hongsheng |
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| Institution: | 1. Fisheries College, Ocean University of China, Qingdao 266003, China;2. Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;3. Shandong Oriental Ocean Sci-Tech |
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| Abstract: | Temperature has a significant effect on the growth, reproduction, distribution and abundance of sea cu-cumbers (Apostichopus japonicus). The suitable temperature range for this species is 5–20℃, and the optimum growth range is 10–16℃, which fluctuates depending on size. When the water temperature exceeds the optimal range,A. japonicus stops feeding and goes into aestivation. In recent years, as global warming has intensified, slow growth, malformation and high mortality of marine animals have been observed frequently because of high temperatures. High seawater temperatures during summer 2013 resulted in significant economic losses in theA. japonicus aquaculture industry in China. Therefore, breedingA. japonicus resistant to high temperatures is re-quired urgently. In this study, we used 1-year-old juveniles (13.6 g±1.8 g) from a directed breeding population (F3 group) and a wild population (C group) as the experimental animals. High temperatures as in ponds in summer were imitated (25℃, 27℃, 29℃, 31℃, 32℃ and 33℃, increasing by 0.5℃ every 12 h). The activities of superoxide dismutase (SOD), catalase (CAT), acid phosphatase (ACP), alkaline phosphatase (AKP), lysozyme (LSZ) and total antioxidant capacity (T-AOC) ofA. japonicus in F3 and C groups were determined and compared after 12 h ther-mal stimulus at different temperatures. The results showed that temperature had a significant influence on the SOD, CAT, ACP, AKP and LSZ activities, and T-AOC. The SOD activities ofthe F3 and C groups initially showed an upward trend, but declined as the temperature increased from 25℃ to 33℃. The activity of SOD in the F3 group was significantly higher than in group C (P<0.05) at 31℃ and 32℃.The CAT activity and T-AOC declined in both groups as temperature increased from 25℃ to 33℃. The CAT activity in the F3 group was higher than in group C at every temperature level, and the difference was significant when the temperature was 27℃, 29℃, 31℃ and 33℃(P<0.05). The T-AOC in group F3 was significantly higher than in group C (P<0.05) at temperature ranges from 25℃ to 33℃. The ACP and AKP activities in both groups declined as temperature increased; the ACP activity in group F3 was significantly higher than in group C (P<0.05), except at 31℃; and the AKP activity in group F3 was sig-nificantly higher than in group C (P<0.05) when the temperature was 25℃, 27℃ and 31℃. The LSZ activity showed an initial upward trend and then declined as the temperature increased in both groups; the activity in the F3 group was significantly higher than in group C at 27℃, 29℃ and 31℃. The results indicated that the immune capacity of thermotolerantA. japonicusto high-temperature stress has improved after three generations of directed breeding. This study provides useful information for further research on selective breeding ofA. japonicus. The rate of global warming is increasing, and the average temperature of the earth’s surface is predicted to rise by 1.5–6.0℃ by the end of the present century, according to the 2007 report by the Intergovernmental Panel on Cli-mate Change (IPCC). Therefore, biological studies of organisms, includingA. japonicus, under high-temperature stress are urgently required. |
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| Keywords: | Apostichopus japonicus thermotolerance antioxidant enzyme hydrolase lysozyme |
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