| '寒富'苹果自花结实性分析及S基因型鉴定 |
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| 引用本文： | 白松龄,伊凯,刘国成,韩振海,许雪峰,王冬梅,李天忠.'寒富'苹果自花结实性分析及S基因型鉴定[J].园艺学报,2008,35(4):475-480. |
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| 作者姓名： | 白松龄 伊凯 刘国成 韩振海 许雪峰 王冬梅 李天忠 |
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| 作者单位： | （中国农业大学园艺植物研究所，北京 100094；辽宁省果树研究所，辽宁熊岳 115009；3沈阳农业大学园艺学院，辽宁沈阳 110161） |
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| 基金项目： | 国家自然科学基金，北京市重点实验室果树逆境生理与分子生物学实验室项目 |
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| 摘 要： | 本研究以苹果‘寒富’品种及其亲本为试材，研究其自交亲和性并鉴定其S基因型。田间授粉试验结果显示，‘寒富’品种表现为自花结实，其母本‘东光’品种亦自花结实；而父本‘富士’品种自花授粉结实率极低，初步推断‘寒富’品种的自花结实性可能遗传自其母本‘东光’。利用PCR技术鉴定'寒富'品种S基因型为S1S9。由于‘东光’品种存在同名异物问题，本研究除日本‘东光’品种外，对辽宁省果树研究所、吉林省果树研究所、沈阳农业大学等单位保存的中国'东光'品种的S基因型进行了鉴定，发现除辽宁省果树研究所保存的'东光'品种S基因型为S2S19，与‘寒富’品种无亲缘关系外，其它两个单位保存的'东光'品种中均存在S9基因型，可能为'寒富'杂交育种时的原始母本。
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| 关 键 词： | 苹果 ‘寒富’ 自花结实性 S基因型 鉴定 |
The Self-fruitfulness of 'Hanfu' Apple and Identification of the S-Genotypes |
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| BAI Song-ling,YI Kai,LIU Guo-cheng,HAN Zhen-hai,XU Xue-feng,Wang Dong-mei,LI Tian-zhong.The Self-fruitfulness of 'Hanfu' Apple and Identification of the S-Genotypes[J].Acta Horticulturae Sinica,2008,35(4):475-480. |
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| Authors: | BAI Song-ling YI Kai LIU Guo-cheng HAN Zhen-hai XU Xue-feng Wang Dong-mei LI Tian-zhong |
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| Institution: | （Institute of Horticultural Plants，China Agricultural University, Beijing 100094, China；Liaoning Research Institute of Pomology, Xiongyue, Liaoning 115009, China；College of Horticulture，Shenyang Agricultural University, Shenyang, Liaoning 110161, China） |
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| Abstract: | 'Hanfu' Apple cultivar(Malus×domestica) and its parents were used in this research to study its self-compatibility and identify their S-genotypes. The pollination test in the field showed that the 'Hanfu' is self-compatible, and its female parent 'Dongguang' is also self-compatible, but its male parent 'Fuji' is self-sterile as known. The S-genotype of 'Hanfu' was identified as S1S9 by the PCR-based method. Also, the S-genotypes of all 'Dongguang' cultivars which are homonym were identified except the 'Toko' which came from Japanese and was named as 'Dongguang' in Chinese. The results showed the S-genotype of 'Dongguang' from Liaoning Research Institute of Pomology is S2S19, showing no relationship with 'Hanfu', and the S-Genotypes of 'Dongguang' cultivars from Shenyang Agricultural University, and Jilin Research Institute of Pomology contain S9 allele, suggesting they are probably the female parent of 'Hanfu'. |
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| Keywords: | Apple Hanfu Self-fruitfulness S-genotype Identification |
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