Application of Functional Markers to Identify Genes for Bacterial Blight Resistance in Oryza rufipogon |
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| Authors: | XIA Zhi-hui HAN Fei GAO Li-fen YUAN Qian-hua ZHAI Wen-xue LIU Di LUO Yue-hua |
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| Institution: | [1]College of Agriculture, Hainan University, Haikou 570228, China [2]Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [3]Institute of Food Crops, Hainan Academy of Agricultural Sciences, Haikou 571100, China |
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| Abstract: | Field resistances of nine accessions of common wild rice (Oryza rufipogon Griff.) and one rice variety (IR24) were evaluated by using nine strains of bacterial blight pathogen (Xanthomonas oryzae pv. oryzae) from the Philippines. IR24 was highly susceptible to all the strains, and six common wild rice accessions resisted all the nine strains, with a resistance frequency of 67%. The accessions Yulin and Wanning were only susceptible to PXO280 and PXO71, respectively. The accession Gaozhou was susceptible to the three strains PXO79, PXO99 and PXO339, whereas resistant to the other six strains. It could be concluded that there is at least one resistance gene in each common wild rice accession. The functional markers of the genes xa5, xa13, Xa21 and Xa27 were used to detect the presence of these resistance genes in the nine tested wild rice accessions, and it was found that four wild rice accessions contained heterozygous xa13. Among the nine common wild rice accessions, five were homozygous for Xa27 and three homozygous for xa27, and the accession Laibin contained neither xa27 nor Xa27. In addition, there were no xa5 and Xa21 in all of these accessions. |
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| Keywords: | Oryza rufipogon bacterial blight resistance gene functional marker |
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