| 香蓼带芽茎段的组织培养和快速繁殖 |
| |
| 引用本文： | 刘晓东,刘 娇,何 淼.香蓼带芽茎段的组织培养和快速繁殖[J].草业科学,2014,31(1):89-94. |
| |
| 作者姓名： | 刘晓东 刘 娇 何 淼 |
| |
| 作者单位： | 东北林业大学园林学院, 黑龙江 哈尔滨 150040 |
| |
| 摘 要： | 以香蓼(Polygonum viscosum)带芽茎段为外植体, MS为基本培养基, 研究了不同浓度的激素对腋芽诱导、腋芽增殖和生根的影响, 从而初步建立了香蓼组织培养快速繁殖体系。结果表明, 诱导腋芽的最适宜培养基为MS+2.0 mg·L-16-BA+0.1 mg·L-1NAA, 诱导率为88.89%;腋芽增殖的最适培养基为MS+2.0 mg·L-1 6-BA+0.3 mg·L-1NAA+0.5 mg·L-1 KT, 增殖倍数为5.17;生根的最适培养基为1/2 MS+0.7 mg·L-1NAA, 生根率高达98.89%, 移栽后植株生长良好, 成活率较高, 达93.33%。该结果为香蓼快繁和基因工程研究提供了新的数据支持。
|
| 关 键 词： | 香蓼 组织培养 带芽茎段 快速繁殖 |
| 收稿时间： | 2013-03-29 |
Tissue culture and rapid propagation of Polygonum viscosum stems stems with axillary buds |
| |
| LIU Xiao-dong,LIU Jiao,HE Miao.Tissue culture and rapid propagation of Polygonum viscosum stems stems with axillary buds[J].Pratacultural Science,2014,31(1):89-94. |
| |
| Authors: | LIU Xiao-dong LIU Jiao HE Miao |
| |
| Institution: | Landscape Architecture College of Northeast Forestry University, Harbin 150040, China |
| |
| Abstract: | In order to establish the tissue culture and rapid propagation system of Polygonum viscosum, the present study explored the impacts of different concentrations of hormone on axillary buds induction, axillary buds proliferation and root induction with the stems with axillary buds of P.m viscosum as explant and MS as the basic medium. The results showed that the optimal medium for axillary bud induction was MS medium with 2.0 mg·L-1 6-BA purine and 0.1 mg·L-1 NAA which had the highest induction rate (88.89%). The optimal medium for axillary buds proliferation was MS medium with 2.0 mg·L-1 6-BA, 0.3 mg·L-1 NAA acid and 0.5 mg·L-1 KT which had the highest coefficient of multiplication (5.17). The optimal medium for root induction was 1/2 MS medium with 0.7 mg·L-1 NAA which had the highest root induction rate (98.89%).Plants grew very well after transplanting with the survival rate of 93.33%.This research supplied favorable information for P.viscosum rapid propagation and genetic engineering. |
| |
| Keywords: | Polygonum viscosum tissue culture stems with axillary buds rapid propagation |
|
| 点击此处可从《草业科学》浏览原始摘要信息 |
| 点击此处可从《草业科学》下载免费的PDF全文 |
|
