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| 红松愈伤组织诱导的初步研究 | |
| 引用本文： | 曹焱,沈海龙,李京,邢亚娟.红松愈伤组织诱导的初步研究[J].林业科技,2009,34(3):1-4. |
| 作者姓名： | 曹焱 沈海龙 李京 邢亚娟 |
| 作者单位： | 1. 东北林业大学,黑龙江啥尔滨150040;黑龙江林业科学研究所,哈尔滨150081 2. 东北林业大学,黑龙江啥尔滨,150040 3. 黑龙江林业科学研究所,哈尔滨,150081 4. 黑龙江大学,哈尔滨,150080 |
| 摘 要： | 以未成熟和成熟合子胚为外植体进行了红松愈伤组织的诱导，其结果表明，成熟胚的诱导效果明显好于幼胚；鉴于诱导产生褐化的主要部位来自胚根，所以外植体应选用子叶、胚轴或去除胚根的胚；诱导培养基采用LM＋2，4-D2．0mg／L＋6-BA0．5mg／L可诱导出较大量Ⅱ型有效愈伤组织，经组织学观察，可见早期原胚团，疑似胚性愈伤组织；继代增殖培养基可采用LM＋2．4-D0．5mg／L＋6-BA0．2mg,／L。 |
| 关 键 词： | 红松 愈伤组织诱导 合子胚外植体 |
Primary Study on Callus Induction of Pinus koraiensis |
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| CAO Yan.Primary Study on Callus Induction of Pinus koraiensis[J].Forestry Science & Technology,2009,34(3):1-4. | |
| Authors: | CAO Yan |
| Institution: | Northeast Forestry University;Heilongjiang Harbin 150040 |
| Abstract: | Callus induction of Pinus koraiensis was studied by taking immature and mature zygotic embryos as explants.The results showed that:(1)mature zygotic embryos were more suitable for callus induction than immature zygotic embryos;(2)explant browning occurred mainly on radicle,therefore cotyledon,hypocotyls and embryo without radicle should be selected as explants;(3)effective type II callus can massively regenerated on LM medium plus 2.0 mg/L 2,4-D and 0.5 mg/L 6-BA,and early pre-embryo and quasi-embryogenic c... |
| Keywords: | Pinus koraiensis Callus induction Zygotic embryo explants |
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