| 象草幼穗离体培养植株再生研究 |
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| 引用本文： | 钟小仙,佘建明,顾洪如,张建丽,倪万潮.象草幼穗离体培养植株再生研究[J].草业学报,2007,16(3):43-48. |
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| 作者姓名： | 钟小仙 佘建明 顾洪如 张建丽 倪万潮 |
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| 作者单位： | 江苏省农业科学院畜牧研究所,江苏,南京,210014;江苏省农业科学院农业生物遗传生理研究所,江苏,南京,210014 |
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| 基金项目： | 科技部农业科技成果转化基金
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江苏省农业三项工程项目 |
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| 摘 要： | 以象草幼穗为外植体材料、改良的MS为基本培养基,研究了不同外源激素组合对不同发育时期幼穗愈伤组织诱导和植株再生能力的影响.结果表明,象草幼穗离体培养最适长度为2～5 cm;愈伤组织诱导培养基中添加4.0 mg/L 2,4-D 0.05 mg/L KT和4.0 mg/L 2,4-D 0.10 mg/L KT时,颗粒状愈伤组织诱导率分别为79.0%和72.6%;转入添加3.0 mg/L 2,4-D 0.2 mg/L 6-BA的继代培养基,分别有40.9%和74.0%的愈伤组织保持颗粒状结构;在添加2.0 mg/L CPPU 0.01 mg/L NAA和0.50 mg/L KT 0.5 mg/L IAA的分化培养基上,经过继代培养后的颗粒状愈伤组织的成苗率分别为36.4%和38.5%,总成苗率达50.2%和43.9%.3片叶大小的幼苗转入附加NAA 0.50 mg/L的1/2 MS壮根培养基,试管苗移栽成活率达95%以上.在继代培养过程中逐代选择外表呈白色、干燥、紧密、颗粒状的愈伤组织,是提高其植株再生能力的有效方法.
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| 关 键 词： | 象草 幼穗 离体培养 愈伤组织 植株再生 |
| 文章编号： | 1004-5759（2007）03-0043-06 |
| 收稿时间： | 2006-05-09 |
| 修稿时间： | 2006-05-09 |
Study on techniques of plant regeneration from immature inflorescences of Pennisetum purpureum in vitro |
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| ZHONG Xiao-xian,SHE Jian-ming,GU Hong-ru,ZHANG Jian-li,NI Wan-chao.Study on techniques of plant regeneration from immature inflorescences of Pennisetum purpureum in vitro[J].Acta Prataculturae Sinica,2007,16(3):43-48. |
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| Authors: | ZHONG Xiao-xian SHE Jian-ming GU Hong-ru ZHANG Jian-li NI Wan-chao |
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| Institution: | 1. Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2. Institute of Agrobiologie Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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| Abstract: | Immature inflorescences of Pennisetum purpureum were used for explants and basic culture medium(MS) was improved.Effects of development period of immature inflorescences and hormone compositions and rates on callus induction and plant regeneration indicated that 2-5 cm immature inflorescences were the optimum.The frequency of,small,compact pellet callus induction reached 79.0 % and 72.6% in callus induction medium supplemented with 4.0 mg /L 2,4-D + 0.05 mg/L KT and 4.0 mg/L 2,4-D + 0.10 mg/L KT respectively.During subculture,40.9% and 74.0% of the small pellets of callus survived in the basic callus subculture medium and in that with 3.0 mg/L 2,4-D + 0.2 mg/L 6-BA added respectively.The rate of green plant regeneration from small pellet callus from subcultures was 36.4% and 38.5% in the differentiation medium supplemented with 2.0 mg/L CPPU +0.01 mg/L NAA or 0.50 mg/L KT + 0.5 mg/L IAA.Regenerated green plants with three leaves were transferred to root vigour medium(0.50 mg/L NAA added to 1/2 MS basic culture medium).The survival rate of green plants cultured in soil was >95%.It was a simple effective method to overcome the reduction of plant generation by selecting the callus of dry,compact,small pellets at an early stage of generation. |
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| Keywords: | Pennisetum purpureum immature inflorescences in vitrol callus plant generation |
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