Changes in the amount of proteins, glycogen and lipids in porcine oocytes during in vitro meiotic maturation |
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| Authors: | Sueo NIIMURA Hiroko TAKANO Akira ONISHI Misa HOSOE |
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| Institution: | Faculty of Agriculture and; Graduate School of Science and Technology, Niigata University, Niigata-shi and; Developmental Biology Department, National Institute of Agrobiological Sciences, Tsukuba-shi, Japan |
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| Abstract: | Changes in the cytoplasmic inclusions during meiotic maturation were histochemically examined in cultured porcine oocytes. The oocytes contained a small amount of protein and glycogen granules throughout the maturation culture, as well as Sudanophilic lipids composed of small, medium and large droplets. Soon after collection, the amount of Sudanophilic lipid droplets of small and medium size was small and there were 167 ± 11.2 large droplets. After being cultured for 22 h, the number of large lipid droplets decreased remarkably, while the number of small and medium ones increased. There were no differences in the number of Sudanophilic lipid droplets of different sizes between ovulated oocytes and the oocytes cultured for 44 h. The oocytes always contained a large amount of neutral fats and lipoids, but not cholesterols. In the oocytes cultured for 22 h with olomoucine, both the resumption of nuclear maturation and the decrease in the size of the Sudanophilic lipid droplets were inhibited. From the present findings, it appears that the change in the size of the Sudanophilic lipid droplets in the cytoplasm of porcine oocytes is closely related to nuclear maturation. |
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| Keywords: | cytoplasmic inclusion histochemistry lipid droplet meiotic maturation porcine oocyte |
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