Improved quantitative method for isolating Calonectria ilicicola from naturally infested soils |
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| Authors: | Sunao Ochi Akio Nakagawa |
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| Institution: | 1. National Agriculture Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki, 305-8666, Japan
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| Abstract: | We developed an improved procedure for quantitative isolation of Calonectria ilicicola from naturally infested soils. The selective medium contained 20?g l-sorbose, 4?g yeast extract, 12.5?mg flutolanil, 1.5?mg thiabendazole, 40?mg chlortetracycline hydrochloride, 10?mg chloramphenicol, 1?mL tergitol, 20?g agar, and up to 1?L with distilled water. Soil samples are wet-sieved (mesh size 0.250?C0.038?mm) and then surface-sterilized for 30?s with 0.25?% NaClO solution before incubation with this medium. Compared with previously reported methods, this method was more effective for isolating C. ilicicola, and the amount of contamination was decreased. |
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