| 麝致病性大肠杆菌的质粒DNA分析 |
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| 引用本文： | 罗燕,程建国,郑士华,赵翠,李蓓,李敏.麝致病性大肠杆菌的质粒DNA分析[J].安徽农业科学,2009,37(25). |
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| 作者姓名： | 罗燕 程建国 郑士华 赵翠 李蓓 李敏 |
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| 作者单位： | 四川农业大学都江堰分校生物科学系,四川都江堰,611830;四川养麝研究所,四川都江堰,611830 |
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| 基金项目： | 四川省教育厅青年基金项目，四川农业大学青年科技创新基金 |
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| 摘 要： | 目的]将麝致病性大肠杆菌从分子水平上分型,为麝大肠杆菌的分子流行学研究提供基础材料。方法]采用Lysis Triton法对24株麝致病性大肠杆菌进行质粒抽提,并用HindⅢ、EcoRⅠ及BamHⅠ3种内切酶对质粒进行单酶切。结果]质粒得率为91.6%,24株麝致病性大肠杆菌具有相同或相似的质粒图谱。结论]质粒DNA分析为四川养麝场致病性大肠杆菌的分子流行病学提供了科学依据。
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| 关 键 词： | 麝 致病性大肠杆菌 质粒DNA 质粒图谱 |
Plasmid DNA Analysis of Pathogenic Escherichia coli in Musk Deer |
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| LUO Yan et al.Plasmid DNA Analysis of Pathogenic Escherichia coli in Musk Deer[J].Journal of Anhui Agricultural Sciences,2009,37(25). |
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| Authors: | LUO Yan |
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| Institution: | LUO Yan et al (Department of Biological Sciences,Sichuan Agricultural University Dujiangyan Campus,Dujiangyan,Sichuan 611830) |
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| Abstract: | Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer.Method] The Lysis Triton method was used to extract plasmids from 24 pathogenic Escherichia coli in musk deer,then HindⅢ,EcoRⅠ and BamHⅠ were used to conduct endo on plasmids.Result] The yield rate of plasmid was 91.6% and 24 pathogenic Escherichia coli in musk deer had the same or similar plasmid profile.Conclusion] The plasmid DNA analysis provided scientific bases for molecular epidemiology,of pathogenic Escherichia coli in musk deer. |
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| Keywords: | Musk deer Pathogenic Escherichia coli Plasmid DNA Plasmid profile |
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