| 德宏奶水牛DGAT1基因多态性及其与产奶性状的相关性分析 |
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| 引用本文： | 樊月圆,王强,张永云,杨忠,富国文,汤守锟,田建云,李卫真.德宏奶水牛DGAT1基因多态性及其与产奶性状的相关性分析[J].家畜生态,2011(6):27-32. |
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| 作者姓名： | 樊月圆 王强 张永云 杨忠 富国文 汤守锟 田建云 李卫真 |
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| 作者单位： | [1]云南农业大学动物科学技术学院,云南昆明650201 [2]中国人民解放军成都军区昆明总医院干细胞与组织器官工程研究中心,云南昆明650032 [3]云南农业大学农科专业基础实验教学示范中心,云南昆明650201 [4]云南生物制药有限公司,云南昆明650224 [5]德宏州潞西市畜牧兽医局,云南潞西678400 |
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| 基金项目： | 云南省应用基础研究面上项目（2007C057M） |
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| 摘 要： | 探讨德宏奶水牛（摩拉水牛×德宏水牛）DGAT1基因多态性与产奶性状的关系，以期为德宏奶水牛产奶性状标记辅助选择侯选基因的选择提供理论依据。以81头泌乳的德宏奶水牛为研究对象，利用PCR—SSCP方法结合候选基因直接测序法检测DGAT1基因第8外显子及第8内含子区的多态性，并采用最小二乘法分析DGAT1基因多态位点对产奶量、乳脂率及乳蛋白率的影响。结果在德宏奶水牛群体中共检测到CTGG，CCGG和CCGT三种基因型，测序结果显示，在检测的群体中未发现K232A位点的突变，而在DGAT1基因第8内含子的第14位检测到C→T突变，第35位检测到G→T突变。多重比较表明，CCGT基因型对德宏奶水牛的乳脂率有显著影响（P〈0．05）。
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| 关 键 词： | DGAT1基因 SNPs 产奶性状 分子标记 水牛 |
The Polymorphisms of DGAT1 and Its Association with Milk Production Traits in Dehong Milk Buffalo |
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| FAN Yue-yuan,WANG Qiang,ZHANG Yong-yufi,YANG Zhong,FU Guo-wen,TANG Shou-kun,TIAN Jian-yuns,LI Wei-zhen.The Polymorphisms of DGAT1 and Its Association with Milk Production Traits in Dehong Milk Buffalo[J].Ecology of Domestic Animal,2011(6):27-32. |
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| Authors: | FAN Yue-yuan WANG Qiang ZHANG Yong-yufi YANG Zhong FU Guo-wen TANG Shou-kun TIAN Jian-yuns LI Wei-zhen |
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| Institution: | 1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming , Yunnan 650201, China ; 2. Research Center of Stem Cells, Tissue and Organ Engineering, Kunming General Hospital of Chengdu Military District of PLA ,Kunming, Yunnan 650032, China; 3. Teaching Demonstration Center of the Basic Experiments of Agricultural Majors ,Yunnan Agricultural University, Kunming , Yunnan 650201,China; 4. Yunnan Biological Phamaceutical Company, Kunming , Yunnan 650224, China; 5. Animal Husbandry and Veterinary Station of Luxi, Luxi, Yunnan 678400, China) |
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| Abstract: | To reveal the SNPs of DGAT1 gene and evaluate its relationship with milk production traits in Dehong milk buffalo （MurrahXDehong）. 81 Dehong milk buffalo were used to detect the SNPs of DGAT1 gene exon 8 and intron 8 by PCR-SSCP and sequencing technology, and then analyzed its relationship with milk yield, fat percentage and protein perceritage by general linear model （GLM）. Three genotypes CTGG, CCGG and CCGT were explored in DGAT1 gene. Sequencing result displayed that two mutations were detected which were 14 C→T and 35 G→T in intron 8, the K232A locus was not detected in Dehong milk buffalo population. Correlation analysis showed that animals with the CCGT genotype was significant in fat percentage than those with genotypes CTGG and CCGG （P〈0.05）. |
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| Keywords: | DGAT1 gene SNPs milk production traits molecular marker buffalo |
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