Nanomechanical basis of selective gating by the nuclear pore complex |
| |
| Authors: | Lim Roderick Y H Fahrenkrog Birthe Köser Joachim Schwarz-Herion Kyrill Deng Jie Aebi Ueli |
| |
| Institution: | M. E. Müller Institute for Structural Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, Basel 4056, Switzerland. roderick.lim@unibas.ch |
| |
| Abstract: | The nuclear pore complex regulates cargo transport between the cytoplasm and the nucleus. We set out to correlate the governing biochemical interactions to the nanoscopic responses of the phenylalanineglycine (FG)-rich nucleoporin domains, which are involved in attenuating or promoting cargo translocation. We found that binding interactions with the transport receptor karyopherin-beta1 caused the FG domains of the human nucleoporin Nup153 to collapse into compact molecular conformations. This effect was reversed by the action of Ran guanosine triphosphate, which returned the FG domains into a polymer brush-like, entropic barrier conformation. Similar effects were observed in Xenopus oocyte nuclei in situ. Thus, the reversible collapse of the FG domains may play an important role in regulating nucleocytoplasmic transport. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
