| 附子总生物碱的提取工艺研究 |
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| 引用本文： | 刘卓,晏星,徐春霞,袁小红.附子总生物碱的提取工艺研究[J].安徽农业科学,2012,40(9):5199-5201. |
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| 作者姓名： | 刘卓 晏星 徐春霞 袁小红 |
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| 作者单位： | 西南科技大学生命科学与工程学院,四川绵阳,621000;西南科技大学生命科学与工程学院,四川绵阳,621000;西南科技大学生命科学与工程学院,四川绵阳,621000;西南科技大学生命科学与工程学院,四川绵阳,621000 |
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| 基金项目： | 四川省科技支撑项目(2008SZ0082) |
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| 摘 要： | 目的]研究附子总生物碱的提取工艺,优选最佳提取条件。方法]以提取率、总生物碱收率和总生物碱纯度为指标,采用单因素试验法,考察提取溶剂、提取方法、提取温度、粉碎度、料液比、提取时间和提取次数对附子中总生物碱提取率的影响,并优选出最佳提取工艺条件。结果]最佳提取工艺条件为:在15℃条件下,将附子粉按1∶10(g/ml)加入无水乙醇,冷浸3次,每次1 d;在此条件下,浸膏得率、总生物碱收率和总生物碱纯度分别为3.15%、0.64%和20.32%。结论]该方法高效、低毒、经济、操作方便、工艺可控,较适合附子总生物碱的提取和工业化生产。
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| 关 键 词： | 附子 总生物碱 冷浸 |
Extraction Technology of Total Alkaloids from RADIX ACONITI LATERALIS PRAEPARATA |
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| Institution: | LIU Zhuo et al(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621000) |
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| Abstract: | Objective] To determine the optimal extraction technology of alkaloids from the roots of RADIX ACONITI LATERALIS PRAEPARATA.Method] Taking extraction rate,and the purity and yield of total alkaloid as the indices,effects of extraction solvent,extraction method,extraction temperature,extraction time,comminution degree,solid-liquid ratio and extraction times on the extraction rate of total alkaloids were studied.And the optimal extraction technology was selected.Conclusion] The optimal extraction technology was as follows: RADIX ACONITI LATERALIS PRAEPARATA powder was added into anhydrous alcohol with the ratio of 1∶ 10(g/ml)under 15 ℃.After cold leaching for 3 times with each time for 1d,the dry extract rate,extraction rate of total alkaloid,and purity of total alkaloid were 3.15%,0.64% and 20.32%,respectively.Conclusion] This method was efficient,low toxic,economical,convenient and can be used for industrial production. |
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| Keywords: | RADIX ACONITI LATERALIS PRAEPARATA Total alkaloids Cold leaching |
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