| 不同培养基对H9亚型禽流感病毒在MDCK细胞上的增殖效果研究 |
| |
| 引用本文： | 张建伟,史爱华,沈佳,景小冬,章振华,李林,姜北宇.不同培养基对H9亚型禽流感病毒在MDCK细胞上的增殖效果研究[J].安徽农业科学,2012,40(8):4609-4611. |
| |
| 作者姓名： | 张建伟 史爱华 沈佳 景小冬 章振华 李林 姜北宇 |
| |
| 作者单位： | 北京市农林科学院畜牧兽医研究所,北京,100097;北京市农林科学院畜牧兽医研究所,北京,100097;北京市农林科学院畜牧兽医研究所,北京,100097;北京市农林科学院畜牧兽医研究所,北京,100097;北京市农林科学院畜牧兽医研究所,北京,100097;北京市农林科学院畜牧兽医研究所,北京,100097;北京市农林科学院畜牧兽医研究所,北京,100097 |
| |
| 基金项目： | 北京市农林科学院一般项目 |
| |
| 摘 要： | 目的]比较3种培养基对H9亚型禽流感病毒在MDCK细胞上的增殖效果。方法]采用DMEM+10%血清、低血清培养基(MEM-MD-611)、无血清培养基(SFE4Mega)3种培养基制备MDCK细胞单层,分别接种不同稀释度的H9亚型禽流感病毒。然后,分别加入含10μg/ml胰蛋白酶的3种培养基作为维持液,培养病毒,每隔24 h观察其细胞病变,并测定上清HA滴度。结果]在培养72～96 h时,无血清培养基病毒液的HA滴度高于低血清培养基,而低血清培养基则高于含血清培养基。结论]3种培养基均可用于制备禽流感病毒抗原,其中无血清培养基、低血清培养基更适用于流感病毒抗原的制备。
|
| 关 键 词： | 培养基 H9亚型流感病毒 MDCK细胞 增殖效果 |
Proliferation of Avian Influenza Virus H9 Subtype in MDCK Cells in Different Culture Media |
| |
| Institution: | ZHANG Jian-wei et al(Institute of Animal Husbandry and veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097) |
| |
| Abstract: | Objective]To screen the best culture media for proliferation of avian influenza virus(AIV) H9 subtype in MDCK cells.Method]The MDCK monolayer cells were respectively cultured in DMEM containing 10%(V/V) newborn calf serum(NBS),low-serum culture medium(MEM-MD-611) and serum-free medium(SFE4Mega) and then inoculated with different dilutions of AIV H9 subtype.These three kinds of media containing 10 μg/ml trypsin were also used for maintenance culture of AIV.Every 24 h,cytopathic changes were observed and the HA titer of the culture supernatant was also determined.Result]After 72-96 h of culture,the HA titers of the culture supernatant derived from the serum-free medium,low-serum culture medium and serum containing medium were in a decreasing manner.Conclusion]The DMEM containing 10%(V/V) newborn calf serum(NBS),low-serum culture medium(MEM-MD-611) and serum-free medium(SFE4Mega) can be used for proliferation of AIV,and the latter two may be more suitable to prepare AIV culture. |
| |
| Keywords: | Culture medium Avian influenza virus H9 subtype MDCK cell Proliferation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
