| 不同基原的郁金类药材中郁金多糖的含量测定 |
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| 引用本文： | 王晓华,朱华,陈旭,曾建红,卢婉飞.不同基原的郁金类药材中郁金多糖的含量测定[J].安徽农业科学,2012,40(9):5173-5174. |
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| 作者姓名： | 王晓华 朱华 陈旭 曾建红 卢婉飞 |
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| 作者单位： | 成都中医药大学,四川成都610075;桂林医学院,广西桂林541004;成都中医药大学,四川成都610075;广西中医学院,广西南宁530001;桂林医学院,广西桂林,541004 |
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| 基金项目： | 广西自然基金项目,任务书(2011GXNSFB018091) |
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| 摘 要： | 目的]研究不同基原的郁金类药材中郁金多糖的含量,为郁金类药材的质量控制提供依据。方法]采用热水浸提法提取郁金粗多糖,经过纯化得到郁金多糖,然后采用分光光度法,经苯酚-硫酸显色后,在489 nm波长处测定其总糖、还原糖及多糖的含量。结果]不同基原的郁金中多糖的含量不同;3个不同基原的郁金药材中,以绿丝郁金多糖含量最大,其多糖的平均含量为25.775%;桂郁金的次之,其多糖含量为3.955%;黄丝郁金的多糖含量最低,为2.695%。结论]该方法操作简单,稳定性好,可用于郁金中多糖的含量测定。
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| 关 键 词： | 郁金(RADIX CURCUMAE) 多糖 含量测定 |
Determination of Polysaccharide Contents in the Different Base Resources of RADIX CURCUMAE |
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| Institution: | WANG Xiao-hua et al(Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan 610075) |
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| Abstract: | Objective] To study the polysaccharide contents in different base recourses of RADIX CURCUMAE,and to provide references for the quality control of RADIX CURCUMAE.Method] The crude polysaccharide was extracted by hot water extraction;after using the spectrophotometry and phenol-sulfuric method,the contents of total sugar,reducing sugar and polysaccharide in the purified polysaccharide were detected at 489 nm.Result] Polysaccharide content varied in different base recourses of RADIX CURCUMAE.Among the 3 different base resources of RADIX CURCUMAE,Curcuma phaeocau lis Val.had the maximum polysaccharide content(25.775%),followed with Curcuma kwangsiensis S.G..Lee et C.F.Liang(3.955%);and Curcuma longa.L.had the minimum polysaccharide content(2.695%).Conclusion] This method was simple and stable and could be used for the content determination of polysaccharide in RADIX CURCUMAE. |
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| Keywords: | RADIX CURCUMAE Polysaccharides Content determination |
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