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| 猪Ghrelin基因的克隆及原核表达 | |
| 引用本文： | 杨连玉,杨文艳,赵颖彩,钱剑,王哲.猪Ghrelin基因的克隆及原核表达[J].中国兽医学报,2005,25(6):614-616. |
| 作者姓名： | 杨连玉 杨文艳 赵颖彩 钱剑 王哲 |
| 作者单位： | 1. 吉林大学,畜牧兽医学院,吉林,长春,130062;吉林农业大学动物科技学院,吉林,长春,130118 2. 吉林大学,畜牧兽医学院,吉林,长春,130062 3. 吉林农业大学动物科技学院,吉林,长春,130118 4. 塔里木农垦大学动物科技学院,新疆,阿克苏,830000 |
| 基金项目： | 国家自然科学基金资助项目（30170713）;吉林省教育厅资助项目（200401） |
| 摘 要： | 从猪下丘脑、胃等组织中提取总RNA，根据已发表的猪的Ghrelin mRNA序列设计合成引物，通过RT-PCR进行cDNA扩增，获得了282bp的片段。将该片段克隆于pMD-18T载体后进行序列分析，确认PCR产物为Ghrelin cDNA。从阳性克隆中提取质粒，经NheⅠ和XhoⅠ双酶切，回收282bp的目的片段，定向克隆到pET-28a表达载体中，提取质粒并再次转化到BL21（DE3）中，成功地筛选出阳性克隆。经IPTG诱导阳性菌，通过SDS-PAGE检测出猪Ghrelin基因的表达． |
| 关 键 词： | Ghrelin 基因克隆 原核表达 猪 |
| 文章编号： | 1005-4545(2005)06-0614-03 |
| 收稿时间： | 2004-03-15 |
| 修稿时间： | 2004年3月15日 |
Chemical Synthesis and Prokaryotic Expression of Ghrelin of Pig |
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| YANG Lian-yu,YANG Wen-yan,ZHAO Ying-cai,QIAN Jian,WANG Zhe.Chemical Synthesis and Prokaryotic Expression of Ghrelin of Pig[J].Chinese Journal of Veterinary Science,2005,25(6):614-616. | |
| Authors: | YANG Lian-yu YANG Wen-yan ZHAO Ying-cai QIAN Jian WANG Zhe |
| Abstract: | Fourty eight strains were isolated from diseased pigs in Qinhuangdao and Tangshan of Hebei province from 2003 to 2004. The morphological,cultural and biochemistry characters indicated that the isolates belonged to genus Streptococcus. Furthermore,43 isolates could be agglutinated by the Lancefield group A-G latex diagnostic kit. The positive samples were 23, 18,1 and 1 strains for group D,C,B and A respectively. But the rest five serums could not be typied. The isolates were demonstrated to be pathogenicity to mouse,rabbit and pig. The results showed that swine Strep. suis were mainly caused by groups D in these area. |
| Keywords: | swine Streptococcus isolate grouping identification |
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