| 免疫亲和色谱高效液相色谱法检测鲟鱼肌肉中常山酮的残留 |
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| 引用本文： | 侯亚莉,郭利敏,丁平,苏亮.免疫亲和色谱高效液相色谱法检测鲟鱼肌肉中常山酮的残留[J].饲料工业,2007,28(24):56-58. |
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| 作者姓名： | 侯亚莉 郭利敏 丁平 苏亮 |
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| 作者单位： | 重庆市饲料兽药监察所,401147,重庆市渝北区黄泥磅黄龙路78号;重庆市饲料兽药监察所,401147,重庆市渝北区黄泥磅黄龙路78号;重庆市饲料兽药监察所,401147,重庆市渝北区黄泥磅黄龙路78号;重庆市饲料兽药监察所,401147,重庆市渝北区黄泥磅黄龙路78号 |
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| 摘 要： | 试验建立了由免疫亲和色谱-高效液相色谱方法检测鲟鱼肌肉中常山酮的残留。样品经胰蛋白酶酶解,乙酸乙酯提取,再用0.125mol/l的醋酸铵缓冲液分离,经免疫亲和色谱柱净化,以乙腈-乙酸铵缓冲液-水为流动相(含0.1%三乙胺),反相液相色谱-紫外检测法检测,检测波长243nm。在20～200μg/kg添加浓度的范围内,样品回收率分别为75.4%～82.0%,变异系数分别为0.7%～8.6%。方法检测限为10μg/kg。结果显示:本方法灵敏度高,干扰少,重复性好。
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| 关 键 词： | 免疫亲和柱 高效液相色谱 常山酮 鲟鱼 残留 |
| 收稿时间： | 2007-10-29 |
| 修稿时间： | 2007年10月29 |
Determination of halofuginone in sturgeon muscle using immunoaffinity column and HPLC |
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| Hou Yali,Guo Limin,Ding Ping,Su Liang.Determination of halofuginone in sturgeon muscle using immunoaffinity column and HPLC[J].Feed Industry,2007,28(24):56-58. |
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| Authors: | Hou Yali Guo Limin Ding Ping Su Liang |
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| Abstract: | A high performance liquid chromatography (HPLC) method had been developed to determine halofuginone in sturgeon muscle. Halofuginone was extracted from trypsin digested tissues as a free base into ethyl acetate and partitioned into ammonium acetate buffer, purified by immunoaffinity chromatography column(IAC), The halofuginones are separated by HPLC and measured by ultraviolet detector at 243 nm with acetonitrile ammonium acetic buffer-water (containing 0.1% triethylamine) as the mobile phase. Recoveries of Halofuginone from samples fortified at 20～200 μg/kg levels ranged 74.6%～81.1%, with coefficients of variation of 0.7%～8.6%. The limit of detection was 10 μg/kg. The procedure provides a rapid, reliable and sensitive method for determination of halofuginone in sturgeon muscle. |
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| Keywords: | IAC HPLC halofuginone sturgeon muscle residue |
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