| 海南龙血树基因组DNA提取方法比较 |
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| 引用本文： | 董美超,唐燕琼,莫饶,尹俊梅,张影波,陈媚,刘迪发.海南龙血树基因组DNA提取方法比较[J].中国农学通报,2010,26(11):28-31. |
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| 作者姓名： | 董美超 唐燕琼 莫饶 尹俊梅 张影波 陈媚 刘迪发 |
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| 作者单位： | 1. 海南大学农学院,海南儋州,571737
2. 中国热带农业科学研究院热带作物品种资源研究所,海南儋州,571737 |
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| 基金项目： | 农业部热带作物种质资源利用重点开放实验室;海南省自然科学基金 |
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| 摘 要： | 摘 要：为选择一种简单、快速、有效的海南龙血树总DNA的提取方法，分别采用CTAB法、SDS法、SDS-CTAB法和改良CTAB法提取海南龙血树嫩叶片的总DNA，用琼脂糖凝胶电泳、紫外分光光度计和ISSR对所提取的总DNA进行检测。结果表明，改良CTAB法提取的DNA A260/A280在1.7-1.9之间，纯度高、杂质少、DNA完整性好。以该方法提取的总DNA为模板进行ISSR扩增，谱带清晰，多态性、稳定性、重复性好。该法提取的总DNA适用于PCR扩增和其他分子生物学研究是有效提取海南龙血树基因组的方法。
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| 关 键 词： | 海南 海南 橡胶树 寒害 |
| 收稿时间： | 2009-12-28 |
| 修稿时间： | 2010-01-08 |
A Comparative Study on Genomic DNA Extraction from Young Leaves of Dracaena cambodiana Pierre ex Gagnepain |
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| Dong Meichao,Tang Yanqiong,Mo Rao,Yin Junmei,Zhang Yingbo,Chen Mei,Li.A Comparative Study on Genomic DNA Extraction from Young Leaves of Dracaena cambodiana Pierre ex Gagnepain[J].Chinese Agricultural Science Bulletin,2010,26(11):28-31. |
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| Authors: | Dong Meichao Tang Yanqiong Mo Rao Yin Junmei Zhang Yingbo Chen Mei Li |
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| Institution: | 1College of Agriculture, Hainan University, Danzhou Hainan 571737;
2Tropical Crops Genetic Resources Institute of Chinese Academy of Tropical Agricultural Sciences, Danzhou Hainan 571737 |
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| Abstract: | To find a simple, rapid and effective total DNA extraction method for Dracaena cambodiana Pierre ex Gagnepain, four methods, CTAB, SDS, SDS-CTAB, Improved CTAB method, were compared. The total DNA was detected by agarose electrophoresis, Spectrophotometer and ISSR amplification. The result showed that the A260/A280 of total DNA extracted by the Improved CTAB method ranged from 1.7 to 1.9 with high-purity, low levels of impurity and good integrity. ISSR fragments amplified with DNA template extracted by Improved CTAB method showed clear bands, and good polymorphic, stability and repetition. The Improved CTAB method was effective for total DNA extraction Dracaena cambodiana Pierre ex Gagnepain, which was suitable for PCR amplification and other molecular biology researches. |
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| Keywords: | ISSRzz |
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