| 花生脂质转运蛋白家族基因的克隆与表达分析 |
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| 引用本文： | 赵传志,李爱芹,王兴军,夏晗,苏磊,李长生.花生脂质转运蛋白家族基因的克隆与表达分析[J].花生学报,2009,38(4):15-20. |
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| 作者姓名： | 赵传志 李爱芹 王兴军 夏晗 苏磊 李长生 |
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| 作者单位： | 山东省农业科学院高新技术研究中心,农业部黄淮海作物遗传改良与生物技术重点开放实验室,山东省作物与畜禽品种改良生物重点实验室,山东,济南,250100 |
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| 基金项目： | 国家"863"重大项目，山东省农业科学院创新基金项目 |
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| 摘 要： | 植物脂质转运蛋白（Lipid transfer protein,LTP）作为一类小分子碱性蛋白在脂类物质的运输、生殖发育,抵御不良环境等方面具有重要的作用,此外,LTP还作为一种过敏原存在。通过构建花生未成熟种子全长cDNA文库,克隆到了5类LTP基因,按照编码蛋白分子量的大小命名为AhLTP-11.5,AhLTP-12.8,AhLTP-11.8,AhLTP-8.3和AhLTP-19.2。生物信息学预测表明AhLTP-11.5属于AAI_LTSS蛋白家族的nsLTP1亚族,AhLTP-11.5编码蛋白的N端有26个氨基酸残基的信号肽序列,ProtCompv8.0预测结果显示该蛋白为分泌蛋白,胞外定位。从系统发育树中可以看出,花生的AhLTP-11.5和拟南芥的AthLTP亲缘关系较近。半定量RT-PCR结果表明AhLTP-11.5基因在花生茎、叶、花和种子中均有表达,在根中几乎检测不到 在花生种子的不同发育时期AhLTP-11.5基因在DAP15d时表达量较低,20d以后表达趋于稳定。
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| 关 键 词： | 花生 脂质转运蛋白 基因表达 |
Cloning and Expression Analysis of Lipid-transfer Protein Family Genes in Arachis hypogaea L. |
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| ZHAO Chuan-zhi,LI Ai-qin,WANG Xing-jun,XIA Han,SU Lei,LI Chang-sheng.Cloning and Expression Analysis of Lipid-transfer Protein Family Genes in Arachis hypogaea L.[J].Journal of Peanut Science,2009,38(4):15-20. |
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| Authors: | ZHAO Chuan-zhi LI Ai-qin WANG Xing-jun XIA Han SU Lei LI Chang-sheng |
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| Institution: | (High-Tech Research Center, Shandong Academy of Agri. Sci.; Key Lab. of Crop Genetic Improvement and Biotech., Huanghuaihai, Ministry of Agri., PRC; Key Lab. for Genetic Improvement of Crop, Animal and Poultry of Shandong, Ji＇nan 250100, China) |
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| Abstract: | As a class of small, basic protein, plant lipid transfer protein plays important roles in lipid transportation, plant reproduction and development. It is also an allergen. By constructing a full-length cDNA library derived from immature peanut seeds, five groups of LTP gene were cloned, which were named AhLTP-11.5, AhLTP-12.8, AhLTP-11.8, AhLTP-8.3 and AhLTP-19.2 according to the molecular weight of coding protein. Bioinformatics analysis showed that AhLTP-11.5 belongs to nsLTP1 subfamily of AAI_LTSS family. ProtComp v8.0 results showed that AhLTP-11.5 was a secreted protein and located in the extracellular. The phylogenetic tree showed that AthLTP was closely related to AhLTP-11.5. Semi-quantitative RT-PCR results showed that AhLTP-11.5 expressed in stems, leaves, flowers, seeds while the expression level in root was very low. During seeds development, the expression level of AhLTP-11.5 was low at DAP15 to compare the expression level at DAP20 through DAP45. |
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| Keywords: | Arachis hypogaea L lipid transfer protein gene expression |
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