一株聚β-羟基丁酸(PHB)产生菌的分离纯化及鉴定 |
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引用本文: | 李晓军,赵旭,常思静,景春娥,薛林贵.一株聚β-羟基丁酸(PHB)产生菌的分离纯化及鉴定[J].中兽医医药杂志,2010,29(5):40-43. |
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作者姓名: | 李晓军 赵旭 常思静 景春娥 薛林贵 |
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作者单位: | 1. 西北师范大学,甘肃兰州,730070;甘肃省高等学校招生办公室 2. 兰州交通大学 |
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基金项目: | 铁道部科技研究开发计划No:20102001E,甘肃省科技支撑计划,国家自然基金 |
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摘 要: | 本研究从土样中分离纯化出一株PHB高产菌株P-9,通过对其进行形态学和生理生化反应特征研究,初步鉴定为苏云金芽孢杆菌(Bacillus thuringiensis)。进一步研究P-9菌的生长规律,发现P-9菌生长的延迟期为0-24 h,24 h后进入对数生长期,同时积累PHB,84 h时细胞进入稳定生长期,108 h时PHB产量达到最大,达1.25 g/L。
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关 键 词: | 聚β-羟基丁酸 细菌 分离 纯化 |
Isolation and identification of a strain of bacteria producing PHB |
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Institution: | LI Xiao-jun(Northwest Normal University,Lanzhou Gansu 730070,China) |
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Abstract: | A strain of P-9 bacteria with high produce of PHB was isolated and purified from soil.P-9 was preliminary appraised as a strain of Bacillus thuringiensis by its characteristics of form and physiology and biochemistry reactions.The lag phase,logarithmic phase and stationary phase was 0-24 h,24-84 h and 84 h,respectively.Further research showed that the maximum yield of PHB in P-9 was 1.25 g/L at 108 h. |
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Keywords: | poly-β-hydroxybutyrate bacteria isolation purification |
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