Enhancement of Hsp70 synthesis protects common carp, Cyprinus carpio L., against lethal ammonia toxicity |
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| Authors: | Sung Y Y Roberts R J Bossier P |
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| Institution: | Department of Aquaculture Science, Faculty of Fisheries and Aqua-Industry, Universiti Malaysia Terengganu (UMT), Kuala Terengganu, Malaysia. yeong@umt.edu.my |
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| Abstract: | Exposure to TEX‐OE®, a patented extract of the prickly pear cactus (Opuntia ficus indica) containing chaperone‐stimulating factor, was shown to protect common carp, Cyprinus carpio L., fingerlings against acute ammonia stress. Survival was enhanced twofold from 50% to 95% after exposure to 5.92 mg L?1 NH3, a level determined in the ammonia challenge bioassay as the 1‐h LD50 concentration for this species. Survival of TEX‐OE®‐pre‐exposed fish was enhanced by 20% over non‐exposed controls during lethal ammonia challenge (14.21 mg L?1 NH3). Increase in the levels of gill and muscle Hsp70 was evident in TEX‐OE®‐pre‐exposed fish but not in the unexposed controls, indicating that application of TEX‐OE® accelerated carp endogenous Hsp70 synthesis during ammonia perturbation. Protection against ammonia was correlated with Hsp70 accretion. |
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| Keywords: | Ammonia stress carp Cyprinus carpio Heat shock proteins TEX‐OE® |
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