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黑土环境中乙草胺的微生物降解特征研究
引用本文:冯慧敏,何红波,武叶叶,张旭东,张明.黑土环境中乙草胺的微生物降解特征研究[J].土壤通报,2008,39(2):379-383.
作者姓名:冯慧敏  何红波  武叶叶  张旭东  张明
作者单位:1. 中国科学院,研究生院,北京,100049
2. 中国科学院,沈阳应用生态研究所,辽宁,沈阳,110016
3. 福建农林大学,福建,福州,350002
4. 辽宁大学,辽宁,沈阳,110036
基金项目:国家重点基础研究发展计划(973计划)
摘    要:通过室内恒温(25℃)避光培养试验,研究了黑土环境中乙草胺的微生物降解特征。在适宜水分条件下,将土壤样品分别进行常规、灭菌、选择性抑菌剂加入等处理后培养并测定土壤乙草胺含量和土壤微生物量。结果显示:在灭菌土壤中乙草胺的残留量较未灭菌土壤显著增加,未灭菌土壤中乙草胺残留数量与微生物量变化密切相关,表明微生物活性是影响乙草胺降解的主要因素。适当的水分有益于土壤中微生物生长,从而促进了土壤中乙草胺的降解。加入青链霉素后乙草胺残留量远大于放线菌酮和常规培养,表明细菌比真菌具有更强的降解乙草胺的能力。随着乙草胺的施药量增加,初期微生物量显著降低,是导致乙草胺总降解率下降的主要原因。

关 键 词:乙草胺  降解  微生物量  黑土
文章编号:0564-3945(2008)02-0379-05
修稿时间:2006年12月11

Study on the Microbial Degradation of Acetochlor in Mollisol
FENG Hui-min,HE Hong-bo,WU Ye-ye,ZHANG Xu-dong,Zhang Ming.Study on the Microbial Degradation of Acetochlor in Mollisol[J].Chinese Journal of Soil Science,2008,39(2):379-383.
Authors:FENG Hui-min  HE Hong-bo  WU Ye-ye  ZHANG Xu-dong  Zhang Ming
Abstract:The microbial degradation of acetochlor in mollisol was investigated by laboratory incubation in dark place at 25℃.The treatments included sterilization and selective microbial biocide addition besides the regular incubation and the content of acetochlor in soil samples as well as microbial biomass carbon(MBC) were determined.The results showed that the residue of acetochlor increased greatly in the sterilized soil and meanwhile the residue magnitude was highly relative with soil MBC in the un-sterilized soil,indicating that the microbial activity was the key factor for acetochlor degradation.The acetochlor residue increased significantly in the sample with actidione addition compared to that of benzylpenicillin sodium(Bs) and streptomycin sulfate(Ss) addition and un-sterilized soil,suggesting bacteria were more efficient for acetochlor degradation than fungi.The degradation efficiency decreased with increased addition of acetochlor mainly due to the significant decrease of MBC induced by herbicide at early stage of incubation.The microorganism growth and activities were improved at the appropriate water content and thus the degradation of acetochlor was enhanced.
Keywords:Mollisol  Acetochlor  Degradation  Microbial biomass carbon
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