| 脂质体介导质粒DNA法转染藏鸡成纤维细胞的研究 |
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| 引用本文： | 梁海青,关伟军,岳文斌.脂质体介导质粒DNA法转染藏鸡成纤维细胞的研究[J].山西农业大学学报(自然科学版),2007,27(2):175-177. |
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| 作者姓名： | 梁海青 关伟军 岳文斌 |
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| 作者单位： | 1. 山西农业大学,文理学院,山西,太谷,030801
2. 中国农业科学院,畜牧研究所,北京,100094 |
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| 摘 要： | 采用Lipofectin阳离子脂质体介导质粒DNA转染藏鸡成纤维细胞,通过优化重组质粒转染细胞的各种参数以寻求最佳的转染条件。结果表明:6孔培养板中细胞汇合度达70%~80%时,用2μL脂质体介导1.5μg重组质粒转染10 h即可获得较满意的转染效率。说明Lipofectin能有效介导质粒pEGFP-N3转染藏鸡成纤维细胞,转染率与细胞生长汇合程度、脂质体包被质粒的浓度比例及转染时间直接相关。
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| 关 键 词： | 藏鸡 成纤维细胞 基因转染 质粒DNA 脂质体 |
| 文章编号： | 1671-8151(2007)02-0175-03 |
| 修稿时间： | 2006年11月20 |
Studies on Transfecting Zang Chicken's Fibroblast with Liposome Mediated Plasmid DNA |
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| LIANG Hai-qing.Studies on Transfecting Zang Chicken''''s Fibroblast with Liposome Mediated Plasmid DNA[J].Journal of Shanxi Agricultural University,2007,27(2):175-177. |
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| Authors: | LIANG Hai-qing |
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| Abstract: | In transfecting Zang chicken's fibroblast with lipofectin liposome mediated plasmid DNA,the optimum transfection condition was explored through combinating all kinds of index of recombinant plasmid transfected cells.The results indicated that the highest efficiency was achieved with cultured fibroblast at 70%～80% confluences.The essential transfection conditions for these efficiencies were in coating pEGFP-N3 of 1.5 μg DNA /100 μL with lipofectin 2 μL/100 μL,and exposing the fibroblast to the DNA-lipofectin complexes for 48 hours.This results indicated that lipofectin could effectively transfer expression pEGFP-N3 plasmid into Zang chicken's fibroblast,DNA/lipofectin mixing concentration ratio,and exposure time of fibroblast to DNA lipofectin complexes. |
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| Keywords: | Zang chicken Fibroblast Gene transfection Plasmid DNA Lipofectin |
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