Analyses of genetic diversity in Cuban rice varieties using isozyme, RAPD and AFLP markers |
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| Authors: | Jorge Luis Fuentes Fabio Escobar Alba Alvarez Geraldo Gallego Miriam Cristina Duque Mirle Ferrer Juan Enrrique Deus Joe M Tohme |
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| Institution: | (1) Centro de Estudios Aplicados al Desarrollo Nuclear, Apartado Postal 6122, calle 30, No 502, Esq. 5 |
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| Abstract: | A survey of the genetic diversity among the major cuban rice cultivars was conducted using isozyme, RAPD and AFLP markers.
Polymorphisms were detected for esterases, peroxidases, alcohol dehydrogenases and polyphenoloxidases systems; 21 RAPD primers
and four AFLP primer combinations. Heterozygosity arithmetic mean value (Hav(p)), the effective multiplex ratio (EMR) and the marker index (MI), were calculated for isozyme, RAPD and AFLP markers. The
mean value of genetic similarity among the different varieties was 0.92 for isozyme, 0.73 for RAPD and 0.58 for AFLP analyses.
Thus, AFLP were able to detect polymorphisms with higher efficiency than RAPD (+15%) and isozyme (+34%). Data from the isozyme,
RAPD and AFLP analyses were used to compute matrices of genetic similarities. The efficiency of the UPGMA for the estimation
of genetic relatedness among varieties was supported by cophenetic correlation coefficients. The resulting values indicated
that the distortion level for the estimated similarities was minimal. The correlation coefficients obtained by the Mantel
matrix correspondence test, which was used to compare the cophenetic matrices for the different markers, showed that estimated
values of genetic relationship given for isozyme and RAPD markers (r = 0.89), as well as for AFLP and RAPD markers (r = 0.82)
were properly related. However, AFLP and isozyme data showed only moderate correlation (r = 0.63). Although the genetic variability
found among the different cultivars was low, both RAPD and AFLP markers proved to be efficient tools in assessing the genetic
diversity of rice genotypes.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | AFLP DNA fingerprinting isozymes RAPD rice |
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