| 中品95-5117抗大豆花叶病毒基因源分析 |
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| 引用本文： | 关荣霞,陈玉波,方宏亮,刘硕,腾卫丽,李文滨,王丕武,常汝镇,邱丽娟.中品95-5117抗大豆花叶病毒基因源分析[J].作物学报,2010,36(4):549-554. |
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| 作者姓名： | 关荣霞 陈玉波 方宏亮 刘硕 腾卫丽 李文滨 王丕武 常汝镇 邱丽娟 |
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| 作者单位： | 中国农业科学院作物研究所 / 国家农作物基因资源与遗传改良重大科学工程 / 农业部作物种质资源利用重点开放实验室，北京 100081; 吉林农业大学农学院，吉林长春 130118; 蚌埠医学院，安徽蚌埠 233030; 南昌大学，江西南昌330031; 东北农业大学大豆研究所，黑龙江哈尔滨 150030 |
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| 基金项目： | 国家自然科学基金重大项目，国家高技术研究发展计划(863计划)项目，中国农业科学院作物科学研究所院所基金基本科研业务费专项，国家科技攻关计划项目 |
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| 摘 要： | 中品95-5117和中品95-5383是以中品661为亲本选育的抗东北花叶病毒病3号株系(SMV3)的大豆新品系。中品95-5383抗病基因的SCAR标记已被定位于大豆F连锁群(Chr.13),与抗病基因Rsv1紧密连锁。利用大豆F连锁群的34个对SSR标记引物及与抗病基因紧密连锁的SCAR标记SCN11及Rsv1候选基因标记Rsv1-f/r,对中品95-5117系谱亲本进行检测,结合对SMV3的抗性鉴定结果进行分析,旨在明确抗SMV3基因在系谱中的传递规律,为利用分子标记辅助选择培育抗SMV3新品种提供依据。通过SSR标记分析发现,中品95-5117和中品95-5383与亲本中品661的相似性最高,而与另外一个亲本鲁豆4号关系较远。SCAR标记SCN11检测表明,只有1份材料Mangnolid(F-53)B为感病基因型。系谱的Rsv1-f/r标记分析表明,Williams82是中品95-5117中Rsv1基因的供体亲本。抗病性鉴定发现鲁豆4号高抗SMV3,但它并不携带Rsv1基因。据上述结果推测中品95-5117中不仅含有Rsv1,还具有来自鲁豆4号的抗病基因,证明该品系比其亲本中品661具有对SMV3更强的抗性。
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| 关 键 词： | 大豆 中品95-5117 系谱 SMV |
| 收稿时间： | 2009-08-27 |
Origin Analysis of Resistance Gene to Soybean Mosaic Virus in Soybean Line ICGR95-5117 |
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| GUAN Rong-Xia,CHEN Yu-Bo,FANG Hong-Liang,LIU Shuo,TENG Wei-Li,LI Wen-Bin,WANG Pi-Wu,CHANG Ru-Zhen,QIU Li-Juan.Origin Analysis of Resistance Gene to Soybean Mosaic Virus in Soybean Line ICGR95-5117[J].Acta Agronomica Sinica,2010,36(4):549-554. |
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| Authors: | GUAN Rong-Xia CHEN Yu-Bo FANG Hong-Liang LIU Shuo TENG Wei-Li LI Wen-Bin WANG Pi-Wu CHANG Ru-Zhen QIU Li-Juan |
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| Institution: | 1.National Key Facility for Crop Gene Resource and Genetic Improvement / Key Laboratory of Germplasm Utilization (MOA), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;2.College of Agronomy, Jilin Agricultural University, Changchun 130118, China;3.Bengbu Medical College, Bengbu 233030, China;4.Nanchang University, Nanchang 330031, China;5.Soybean Research Institute, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | Soybean mosaic virus (SMV) is a prevalent viral pathogen of soybean. The soybean mosaic virus N3 (SMV3) is the most toxic strain in northeast China. Soybean lines ICGR95-5117 and ICGR95-5383 with high resistance to SMV3,which were all derived from ICGR661,are recently released from Chinese Academy of Agricultural Sciences. Several genes resistant to soybean mosaic virus have been identified and mapped on different soybean linkage groups by molecular markers. The re-sistance gene in ICGR95-5383 has been mapp... |
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| Keywords: | SMV Soybean ICGR95-5117 Pedigree SMV |
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