| 次氯酸钠用于植物培养基灭菌方法初探 |
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| 引用本文： | 张雨婷,王贤,王永勤.次氯酸钠用于植物培养基灭菌方法初探[J].中国农学通报,2012,28(4):263-268. |
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| 作者姓名： | 张雨婷 王贤 王永勤 |
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| 作者单位： | 1. 北京市第二中学,北京,100010
2. 北京市农林科学院蔬菜研究中心,北京,100097 |
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| 基金项目： | 北京市农林科学院科技创新能力建设专项“蔬菜种质资源收集、评价与创新”（KJCX201101010）：国家自然基金“洋葱异源胞质雄性不育机制解析”（31071794）. |
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| 摘 要： | 对在培养基的配制过程中添加次氯酸钠替代培养基高压蒸汽灭菌的方法进行了研究，取得了如下结果：在培养基凝固后添加次氯酸钠，温度25℃、光照强度2000lx、光照时间为16小时的培养条件下，浓度为360mg/L时，仍有部分培养基污染；在培养基配制过程中，琼脂加热溶解后，趁热在培养基中添加10mg/L次氯酸钠，未发污染。以‘神马’菊花为外植体，在培养基配制过程中加入终浓度20mg/L的次氯酸钠时,植株可以正常生长，而且，生长状况好于高压灭菌组；次氯酸钠浓度高于50 mg/L时，生长状况差于高压灭菌组，而且会产生伤害。
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| 关 键 词： | 半农半牧区 半农半牧区 |
| 收稿时间： | 2011/12/15 0:00:00 |
| 修稿时间： | 2012/1/23 0:00:00 |
Sodium hypochlorite for sterilization Methods of plant culture medium |
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| Zhang Yuting
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Wang Xian
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Wang Yongqin
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Sun Jifan.Sodium hypochlorite for sterilization Methods of plant culture medium[J].Chinese Agricultural Science Bulletin,2012,28(4):263-268. |
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| Authors: | Zhang Yuting Wang Xian Wang Yongqin Sun Jifan |
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| Institution: | 1Beijing No. 2 Middle School, Beijing 100010; 2Belting Vegetable Research Center, Beifing Academy of Agriculture and Forestry Science, Beijing 100097) |
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| Abstract: | AbstSterilization methods in the culture medium of plants were improved so that the time of pressure steam sterilization and energy consumption could be shortened. Different concentration sodium hypochlorite was added to sterilization during the process of making culture medium. The results showed that during the media preparation process, the dissolved agar was heated, 10 mg/L sodium bypochlorite was supplemented into the hot medium, polluted culture medium was not found. With ＇God Ma＇ chrysanthemum explants in culture medium during the preparation of the final concentration 20 mg/L of sodium hypochlorite, all the plants grew normally, furthermore, their growth conditions were better than that of the group with the high-pressure sterilization. When sodium hypochlorite concentrations were above 50 mg/L, the growth was not only poorer than that of the control, but also did harm to them. In these conditions, the proper concentration of sodium hypochlorite replacing high-pressure steam sterilization was effective. This method could shorten the time and energy consumption. |
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| Keywords: | plant tissue culture medium sterilization methods improvement sodium hypochlorite |
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