| 农杆菌介导脂滴包被蛋白基因8-3转化烟草的研究 |
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| 引用本文： | 牛伯庆,汪文静,宋洪英,谢响明.农杆菌介导脂滴包被蛋白基因8-3转化烟草的研究[J].中国农学通报,2012,28(3):240-244. |
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| 作者姓名： | 牛伯庆 汪文静 宋洪英 谢响明 |
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| 作者单位： | 北京林业大学生物科学与技术学院,北京,100083 |
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| 基金项目： | 转基因生物新品种培育重大专项“抗大豆菌核病重要基因克隆及功能验证”（2009ZX08009-089B） |
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| 摘 要： | 为开发新的抗菌核病基因，针对抗病相关的脂滴包被蛋白基因8-3，设计了分别带有酶切位点Xba I和BamH I的一对引物，从克隆载体pMD19-T-8-3中扩增到基因8-3，双酶切8-3并连接到植物表达载体pBI 121上；将重组质粒导入根癌农杆菌(Agrobacterium tumefaciens) LBA4404中，通过其介导将8-3基因转化到烟草中；获得了35株抗性转化株，PCR及测序分析鉴定阳性植株为21株，RT-PCR检测到基因8-3在其中能够表达；通过接种核盘菌和草酸处理试验，表明8-3基因可使烟草提高了草酸耐受水平，从而对菌核病具备一定的抗性。本实验研究了潜在功能基因8-3的抗病性，为植物抗菌核病的研究奠定了基础。
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| 关 键 词： | 台地园 台地园 中国古典园林 景观 |
| 收稿时间： | 2011/10/27 0:00:00 |
| 修稿时间： | 2011/12/12 0:00:00 |
Study of Transformation of Tobacco with Perilipin Gene 8-3 Mediated by Agrobacterium tumefaciens |
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| Niu Boqing
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Wang Wenjing
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Song Hongying
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Xie Xiangming.Study of Transformation of Tobacco with Perilipin Gene 8-3 Mediated by Agrobacterium tumefaciens[J].Chinese Agricultural Science Bulletin,2012,28(3):240-244. |
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| Authors: | Niu Boqing Wang Wenjing Song Hongying Xie Xiangming |
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| Institution: | (College of Biological Sciences and Biotechnology, Beijing Forestry University , Beijing 100083) |
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| Abstract: | To find new resistance genes and study perilipin gene 8-3 related to Sclerotinia sclerotiorum, a couple of primers containing restriction enzyme sites of Xba I and BamH I were designed to clone the 8-3 from cloning vector pMD19-T-8-3, and the product was digested and lignased with plant expression vector pBI121. The pBI121-8-3 was transformed into LBA4404 and the 8-3 gene was transformed into Nicotiana tabacum 89 mediated by Agrobacterium tumefaciens. 35 resistant lines were obtained, 21 of which were identified as transgenic plants by PCR and sequencing. The further identification of RT-PCR was confirmed that the 8-3 was expressed in the transgenic plants. The transgenic tobacco infected with Sclerotinia sclerotiorum and oxalic acid showed that 8-3 could improve the plants＇ tolerance level to oxalic acid and enhance the resistance to Sclerotinia sclerotiorum to a certain extent, which laid the foundation for the study of 8-3 gene and plant resistance to Sclerotinia sclerotiorum. |
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| Keywords: | perilipin transformation mediated by Agrobacterium tumefaciens transgenic tobacco Sclerotinia sclerotiorum resistance |
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