| 不同激素配比对橡胶树叶片愈伤组织诱导的影响 |
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| 引用本文： | 孙爱花,华玉伟,黄天带,周权男,戴雪梅,黄华孙.不同激素配比对橡胶树叶片愈伤组织诱导的影响[J].中国农学通报,2012,28(4):24-27. |
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| 作者姓名： | 孙爱花 华玉伟 黄天带 周权男 戴雪梅 黄华孙 |
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| 作者单位： | 中国热带农业科学院橡胶研究所/国家橡胶树育种中心/农业部橡胶树生物学重点开放实验室,海南儋州,571737 |
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| 基金项目： | 海南省自然科学资金“橡胶树内珠被愈伤组织继代培养及其RAPD分析”（809028）：中国热带农业科学院橡胶研究所基本科研业务费专项项目“橡胶树次级体细胞胚发生的分子机理研究”（1630022011004）. |
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| 摘 要： | 为拓宽橡胶树外植体来源，促进其组织培养技术的发展，本试验以橡胶树无菌苗叶片为外植体材料，采用不同激素及其配比，诱导其叶片愈伤组织的产生。当6-BA浓度一定时，随着NAA浓度的提高，叶片愈伤组织率有逐步提高的趋势，达到1.5 mg/L时，愈伤诱导率最高，而当6-BA浓度>1.5 mg/L时，愈伤诱导率呈现下降的趋势；通过KT与NAA的不同浓度配比组合发现：大多数培养基的愈伤诱导率都低于10%，其中有些还为0；NAA浓度相同时，6-BA的愈伤组织诱导能力明显好于KT。橡胶树叶片愈伤组织的诱导必须依赖于植物激素，在不含激素或单独使用某种激素的培养基中均不能诱导愈伤组织的形成,叶片愈伤组织诱导率最佳培养基为改良MS培养基+1.5 mg/L NAA+1.0 mg/L 6-BA，其诱导率达到26.7%。
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| 关 键 词： | 区域特征 区域特征 |
| 收稿时间： | 2011/9/13 0:00:00 |
| 修稿时间： | 2011/12/19 0:00:00 |
Effects of Different Hormone Combination on Callus Induction of Leaf Explant from Hevea Brasiliensis |
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| Sun Aihua
,
Hua Yuwei
,
Huang Tiandai
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Zhou Quannan
,
Dai Xuemei
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Huang Huasun.Effects of Different Hormone Combination on Callus Induction of Leaf Explant from Hevea Brasiliensis[J].Chinese Agricultural Science Bulletin,2012,28(4):24-27. |
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| Authors: | Sun Aihua Hua Yuwei Huang Tiandai Zhou Quannan Dai Xuemei Huang Huasun |
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| Institution: | (Rubber Research Institute, Chinese Academy of Tropical Agricultural Scienees/State Center for Rubber Breeding/ Key Laboratory of Ministry of Agriculture for Tropical Crop Physiology, Danzhou Hainan 571737) |
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| Abstract: | To widen the explants source and promote the development of tissue culture technology of Hevea brasiliensis. The leaves from sterile seedlings of rubber tree as explants were induced to calla on media containing different concentrations and combinations of plant hormones. When the concentration of 6-BA was definite, the induction rate of callus was gradually improved with the increasing of NAA concentration. The rate was the highest, while the concentration of 6-BA reached 1.5 mg/L, and more than 1.5 mg/L, the rate was gradually declining. The results of different concentrations and combinations of KT and NAA showed that the calla induction rates of most media were less than ten percent, some were even zero. When NAA had the same concentration, the ability of callus induction from 6-BA was obviously superior to KT. The callus induction rate of leaf must depend on plant hormone in Hevea brasiliensis. The calla can not be induced when the media had no hormone or containing single hormone. The modified MS＋1.5 mg/L NAA＋1.0 mg/L 6-BA was the most appropriate medium of callus induction rate from leaf, and the rate reacbed to 26.7%. |
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| Keywords: | Hevea brasiliensis leaf callus plant hormone |
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