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| 拟南芥T1N6_22在抵抗Pst DC3000侵染过程中的功能分析 | |
| 引用本文： | 郝丛丛,郑会欣,贾娇,司贺龙,陈展,赵斌,张靖,邢继红,董金皋.拟南芥T1N6_22在抵抗Pst DC3000侵染过程中的功能分析[J].中国农业科学,2013,46(4):678-685. |
| 作者姓名： | 郝丛丛 郑会欣 贾娇 司贺龙 陈展 赵斌 张靖 邢继红 董金皋 |
| 作者单位： | 1. 河北农业大学生命科学学院真菌毒素与植物分子病理学实验室,河北保定,071001 2. 吉林省农林科学院植物保护研究所,吉林公主岭,136100 3. 河北省农林科学院果树研究所,石家庄,050061 |
| 基金项目： | 国家自然科学基金（31200203)、河北省自然科学基金（C2012204032）、河北农业大学青年科学基金（QJ201231） |
| 摘 要： | 【目的】明确拟南芥抗灰霉病基因T1N6_22在抗Pst DC3000过程中的功能，分析T1N6_22影响拟南芥对Pst DC3000的抗性原因。【方法】对t1n6_22突变体和转基因回复突变体（t1n6_22/T1N6_22）接种Pst DC3000，检测其症状；采用间苯胺蓝染色法检测接种突变体中胼胝质的积累情况；测定接种叶片中Pst DC3000的生长量，明确T1N6_22在拟南芥抗Pst DC3000过程中的功能。利用RT-PCR技术，检测SA、JA和ET对T1N6_22表达的影响及T1N6_22对抗病防御相关基因表达的影响；利用quantitative real-time PCR技术，检测Pst DC3000对T1N6_22及抗病相关基因表达的影响，分析T1N6_22影响拟南芥对Pst DC3000的抗性原因。【结果】t1n6_22突变体接种Pst DC3000后表现明显的抗病症状，而回复突变体t1n6_22/T1N6_22和拟南芥野生型表现明显的感病症状。SA处理拟南芥野生型，T1N6_22的表达量明显增强，经JA和ACC处理，该基因的表达量无显著变化。t1n6_22突变体中，PAL、PR4、PPO、SOD和CAT的表达水平均明显高于野生型Col-0和转基因回复植株。接种Pst DC3000后，拟南芥野生型中T1N6_22及抗病相关基因PR1、PR3、PR5和PDF1.2的表达量明显增强。【结论】T1N6_22在拟南芥抗Pst DC3000过程中起负调控作用，T1N6_22的表达受SA诱导，可能主要通过调节植物的次生代谢产物的分泌影响拟南芥对Pst DC3000的抗性。 |
| 关 键 词： | T1N6_22 拟南芥 Pst DC3000 抗病信号途径 |
| 收稿时间： | 2012-11-05 |
Functional Analysis of T1N6_22 in Arabidopsis thaliana Against the Infection of Pst DC3000 |
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| HAO Cong-Cong,ZHENG Hui-Xin,JIA Jiao,SI He-Long,CHEN Zhan,ZHAO Bin,ZHANG Jing,XING Ji-Hong,DONG Jin-Gao.Functional Analysis of T1N6_22 in Arabidopsis thaliana Against the Infection of Pst DC3000[J].Scientia Agricultura Sinica,2013,46(4):678-685. | |
| Authors: | HAO Cong-Cong ZHENG Hui-Xin JIA Jiao SI He-Long CHEN Zhan ZHAO Bin ZHANG Jing XING Ji-Hong DONG Jin-Gao |
| Institution: | 1.College of Life Science, Agricultural University of Hebei/Mycotoxin and Molecular Plant Pathology Laboratory, Baoding 071001, Hebei; 2.Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin; 3.Institute of Fruit Tree, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050061 |
| Abstract: | 【Objective】 The objective of this study is to investigate the function of the T1N6_22, a resistant gene of Arabidopsis against B. cinerea, in Arabidopsis resistance to Pseudomonas syringae pv. tomato DC3000, to further analyze the mechanism of T1N6_22 genes in Arabidopsis resistance to Pst DC3000. 【Method】 The symptoms, increase of the content of callose and bacterial concentration of the t1n6_22 and t1n6_22/T1N6_22 in plants inoculated with Pst DC3000 were investigated to study the function of the T1N6_22 in Arabidopsis resistance to Pst. DC3000. RT-PCR technology was used to analyze the expression of T1N6_22 in Col-0 treatment with SA, JA, ET and the expression of defence-related genes in Col-0, the t1n6_22 and t1n6_22/T1N6_22 plants. Quantitative real-time PCR technology was used to analyze the expression of T1N6_22 and the key genes involved in the SA, JA/ET signal pathway in Col-0 inoculated with Pst DC3000. 【Result】 The t1n6_22 exhibited enhanced resistance to Pst DC3000, the Col-0 and complemental plants showed an obvious susceptibility to Pst DC3000. The expression of T1N6_22 in Col-0 treatment with SA was significantly enhanced, suggesting the expression of T1N6_22 induced by SA. Compared with the Col-0 and t1n6_22/T1N6_22 plants, the expression of PAL, PR4, PPO, SOD and CAT genes were downregulated in the t1n6_22 plants. The expression of T1N6_22 and the key genes involved in the SA, JA/ET signal pathway, such as PR1, PR3, PR5 and PDF1.2 genes, were upregulated in Col-0 after inoculation of Pst DC3000.【Conclusion】T1N6_22 gene is a negative regulatory component of Arabidopsis against Pst DC3000 and the T1N6_22 gene expression is induced by SA. T1N6_22 may be involved in the regulation of plant secondary metabolites in impact of the resistance to Pst DC3000 in Arabidopsis. |
| Keywords: | T1N6_22 Arabidopsis thaliana Pst DC3000 disease-resistant pathway |
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