| 多叶苜蓿花药培养技术体系的建立 |
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| 引用本文： | 武自念,魏臻武,郑曦.多叶苜蓿花药培养技术体系的建立[J].中国农业科学,2013,46(10):2004-2013. |
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| 作者姓名： | 武自念 魏臻武 郑曦 |
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| 作者单位： | 扬州大学动物科学与技术学院/扬州大学草业科学研究所,江苏扬州,225009 |
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| 基金项目： | 国家“863”（2008AA10Z149）、国家自然科学基金项目（30972136）、江苏省普通高校研究生科研创新计划资助项目（CXZZ11_0991） |
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| 摘 要： | 【目的】建立多叶苜蓿高效花药培养体系，获得再生植株，为苜蓿倍性、基因组学及分子生物学的研究提供基础材料。【方法】以淮阴苜蓿与多叶苜蓿品种飞马（Grandeur）杂交F1的花药为外植体，使用NB+2,4-D（0.5 mg?L-1）+NAA（0.3 mg?L-1）+6-BA（0.5 mg?L-1）+KT（3.0 mg?L-1）研究苜蓿现蕾后花药愈伤组织的诱导能力；利用正交设计L16（45）筛选适宜苜蓿花药愈伤组织分化的培养基；并用1/2B5和1/2MS添加不同浓度的NAA研究适宜的生根培养基。【结果】苜蓿现蕾后15—28 d愈伤组织诱导率达到最高，最高为82.79%。适宜苜蓿花药愈伤组织分化的培养基组合为BⅠ+ CH（200 mg?L-1）+2-IP（0.5 mg?L-1）+ NAA（0.2 mg?L-1）+ KT（1.0 mg?L-1），分化率可达54.05%。不定芽在1/2 B5+NAA（0.3 mg?L-1）培养基上生根效果最好，生根率可达88.46%。【结论】成功建立了苜蓿高效花药培养体系，并获得再生植株。
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| 关 键 词： | 苜蓿 花药培养 培养基 芽的分化 |
| 收稿时间： | 2012-09-28 |
Construction of a Highly Efficient Technology System in Anther Culture of Mutlifoliate Alfalfa |
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| WU Zi-Nian,WEI Zhen-Wu,ZHENG Xi.Construction of a Highly Efficient Technology System in Anther Culture of Mutlifoliate Alfalfa[J].Scientia Agricultura Sinica,2013,46(10):2004-2013. |
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| Authors: | WU Zi-Nian WEI Zhen-Wu ZHENG Xi |
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| Institution: | College of Animal Science and Technique/Institute of Forage and Grassland Science,Yangzhou University, Yangzhou 225009, Jiangsu |
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| Abstract: | 【Objective】 This experiment was carried out to establish an efficient anther culture system of mutlifoliate alfalfa, and get regenerate plantlet. It will also provide basic materials for the research of ploidy, genomics and molecular biology of alfalfa.【Method】 Firstly, Huaiyin alfalfa and a mutlifoliate alfalfa varieties named Grandeur were used as the parents to create a hybrid, and the anther of hybrid F1 was used as the explants. The ability of induction of callus after bud was studied by using the culture medium of NB added with 2,4-D (0.5 mg•L-1), NAA (0.3 mg•L-1) and KT (3.0 mg•L-1). The appropriate medium was screened for the differentiation of anther callus of alfalfa by orthogonal design L16 (45). In addition, the rooting ability from buds was studied by adding different concentrations of NAA into 1/2 B5 and 1/2 MS.【Result】The highest induction rate of alfalfa callus was in 15 to 28 days after budding, and the maximum value was 82.79%. The culture medium that was good for the differentiation of the callus of alfalfa anther was made up of BⅠwith CH (200 mg•L-1), 2-IP (0.5 mg•L-1), NAA (0.2 mg•L-1) and KT (1.0 mg•L-1), and the maximum regeneration rate was 54.05%. The best culture medium for adventitious bud was 1/2 B5 with NAA (0.3 mg•L-1), and the maximum rooting rate was 88.46%.【Conclusion】An efficient anther culture system of mutlifoliate alfalfa was established successfully, and the regenerated plants were obtained. |
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| Keywords: | alfalfa anther culture medium buds differentiation |
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