| 高效液相色谱-串联质谱法检测猪、鸡可食性组织中喹嗯啉类兽药残留标示物 |
| |
| 引用本文： | 董义春,张丽芳,张可煜,袁宗辉.高效液相色谱-串联质谱法检测猪、鸡可食性组织中喹嗯啉类兽药残留标示物[J].中国兽医学报,2010,30(1). |
| |
| 作者姓名： | 董义春 张丽芳 张可煜 袁宗辉 |
| |
| 作者单位： | 1. 华中农业大学,动物医学院,湖北,武汉,430070
2. 中国农业科学院上海兽医研究所,上海,200241 |
| |
| 基金项目： | 上海市科技兴农科技攻关项目，上海市科学技术委员会研发公共服务平台项目，上海市科学技术委员会技术标准专项 |
| |
| 摘 要： | 为检测猪、鸡可食性组织中喹噁啉类兽药残留标示物喹噁啉-2-羧酸(QCA)和3-甲基喹噁啉-2-羧酸(MQ-CA),建立了同时检测这2种残留标示物的高效液相色谱-串联质谱法.将样品碱水解,乙酸乙酯等液-液萃取,65℃氮气吹干,甲醇:0.5%甲酸水溶液(30:70)溶解,0.45μm微孔滤膜过滤后,采用高效液相色谱-串联质谱分析.结果显示,猪、鸡可食性组织中喹噁啉类兽药残留标示物MQCA和QCA检测限为0.2～0034μg/kg和0.5～0.9μg/kg,定量限为0.5～0.61μg/kg和0.77～1.31μg/kg.相对回收率在90.07%～106.8%范围内,日内变异系数≤13.69%.日间变异系数≤15.43%.MQCA和QCA在2～100μg/kg范围内具有较好的线性关系(r~2>0.99).结果表明,本方法简单、灵敏度高.适用于猪、鸡可食性组织中喹噁啉类兽药残留标示物的定量检测.
|
| 关 键 词： | 喹噁啉类 残留标示物 喹噁啉-2-羧酸 3-甲基喹噁啉-2-羧酸 猪 鸡 高效液相色谱-串联质谱法 |
An HPLC/MS-MS method for the determination of marker residues metabolites of quindoxins in edible tissues of swine and chicken |
| |
| DONG Yi-chun,ZHANG Li-fang,ZHANG Ke-yu,YUAN Zong-hui.An HPLC/MS-MS method for the determination of marker residues metabolites of quindoxins in edible tissues of swine and chicken[J].Chinese Journal of Veterinary Science,2010,30(1). |
| |
| Authors: | DONG Yi-chun ZHANG Li-fang ZHANG Ke-yu YUAN Zong-hui |
| |
| Abstract: | A high performance liquid chromatography-tandem mass spectrometry (HPLC/MS-MS) method was de-veloped to simultaneously determine quinoxaline-2-carboxylic acid (QCA) and methyl-3-quinoxaline-2-carboxylic acid (MQCA) that is the marker residues metabolites of quindoxins in edible tissues of swine and chicken. The samples were hydrolyzed with alkaline and extracted by the liquid-liquid method using ethyl acetate,The extraction was dried by nitrogen gas,redissolved in methanol : 0.5% formic acid (30:70) and filtered ration 0.45 μm micropore film. The final solution was analyzed by HPLC/MS-MS. The decision limits and the detection capabilities of MQCA and QCA in liver and muscle of swine and chicken were 0.2-0.34 μg/kg,0.5-0.9 μg/kg,0.5-0.61 μg/kg and 0.77-1. 31μg/kg,respectively. The relative recovery rate were in the range of 90.07%-106.8%, the inter-day variation were less than 13.69% and the intra-day variation were less than 15.43%. The calibration curves were linear (r~2>0.99)in the range of 2-100μg/kg. The method was simple and accurate enough to determine the marker residues metabo-lites of quindoxins in edible tissues of swine and chicken. |
| |
| Keywords: | quindoxins marker residues quinoxaline-2-carboxylic acid methyl-3-quinoxaline-2- carboxylic acid swine chicken H PLC/MS-MS |
| 本文献已被 万方数据 等数据库收录！ |
|
