| 镉对大鼠原代肝细胞的毒性损伤 |
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| 引用本文： | 汪纪仓,张英,裔传卉,邹辉,刘宗平.镉对大鼠原代肝细胞的毒性损伤[J].中国兽医学报,2010,30(4). |
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| 作者姓名： | 汪纪仓 张英 裔传卉 邹辉 刘宗平 |
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| 作者单位： | 1. 扬州大学,兽医学院,江苏,扬州,225009;河南科技大学动物科技学院,河南,洛阳,471003
2. 扬州大学,兽医学院,江苏,扬州,225009 |
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| 基金项目： | 江苏省高校"青蓝工程"中青年学术带头人培养对象资助项目 |
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| 摘 要： | 用两步灌流法获得大鼠肝细胞,肝细胞暴露于浓度为2.5、5、10μmol/L的醋酸镉24 h.测定了细胞活力、培养上清液中乳酸脱氢酶(LDH)、天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)活性及细胞内谷胱甘肽过氧化物酶(GSH-PX)活性、还原型谷胱甘肽(GSH)和丙二醛(MDA)含量的变化.结果表明,细胞相对存活率显著下降(P<0.01),LDH、AST和ALT的释放量增加,5μmol/L和10 μmol/L剂量组与对照组相比差异均显著(P<0.01),细胞内GSH-PX活性降低,各剂量染毒组与对照组相比,差异均极显著(P<0.01);细胞内GSH含量升高,5 μmol/L和10μmol/L剂量组与对照组差异均显著(P<0.05),细胞内MDA含量升高,10μmol/L剂量组与对照组相比差异显著(P<0.05).表明镉可致肝细胞损伤,并且氧化应激起了重要作用.
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| 关 键 词： | 镉 大鼠肝细胞 氧化应激 |
Cytoxicity induced by cadmium in primary cultured rat hepatocytes |
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| WANG Ji-cang,ZHANG Ying,YI Chuan-hui,ZOU Hui,LIU Zong-ping.Cytoxicity induced by cadmium in primary cultured rat hepatocytes[J].Chinese Journal of Veterinary Science,2010,30(4). |
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| Authors: | WANG Ji-cang ZHANG Ying YI Chuan-hui ZOU Hui LIU Zong-ping |
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| Abstract: | Rat hepatocytes were isolated by a two-step perfusion technique.The hepatocytes were incubated with cad-mium acetate (2.5,5 and 10 μmol/L) for 24 h.Cell viability,activities of lactate dehydrogenase (LDH),spartate aminotransferase (AST),alanine aminotransterase (ALT) in cell-free medium and glutathione peroxidaze(GSH-PX) activity,cellular glutathione (GSH) and malondialdehyde (MDA) content in hepatocytes were measured at the end of incubation.The results showed that cell viability was decreased significantly (P<0.01),the activities of LDH,AST and ALT increased in the 5 and 10 μmol/L groups were significantly higher than those of the control group (P<0.01).Different dosages of Cd induced significant elevation of GSH-PX activity (P<0.01).When the doses of Cd were higher than 5 μmol/L,GSH content increased (P<0.05).The content of MDA was increased in the 10 μmol/L groups (P<0.05).It suggests that cadmium can injure hepatocytes and induce oxidative stress. |
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| Keywords: | cadmium rat hepatocytes oxidative stress |
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