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| 4株猪链球菌的病原生物学特性分析 | |
| 引用本文： | 杜华茂,丁玉春,王丽丽,崔志刚,刘佩红,叶长芸,徐建国.4株猪链球菌的病原生物学特性分析[J].中国兽医学报,2010,30(1). |
| 作者姓名： | 杜华茂 丁玉春 王丽丽 崔志刚 刘佩红 叶长芸 徐建国 |
| 作者单位： | 1. 西南大学生物技术学院,重庆,400715;中国疾病预防控制中心,传染病控制所,北京,102200 2. 重庆养猪科学研究院,重庆,402460 3. 中国疾病预防控制中心,传染病控制所,北京,102200 4. 上海市畜牧兽医站,上海,201103 |
| 基金项目： | 中加联合项目，西南大学校科研和教改项目 |
| 摘 要： | 对4株新分离猪链球菌NJ25、SH29、SH40和RC4726进行病原学特性分析,以评估其对公共卫生的威胁性。利用中国疾病预防控制中心传染病所制定的猪链球菌2型鉴定方案,对4株新分离猪链球菌NJ25、SH29、SH40和RC4726进行毒力相关基因检测、细菌染色体酶切片段多态性分析(PFGE)、多位点序列分型(MLST),并用C57BL/6小鼠作致病性试验。结果显示,NJ25株为ST7序列型,SH29、SH40为ST1序列型,3株菌的基因型均为sly+mrp+ef+;RC4726的7个管家基因座位分别为aroA(18)、cpn60(34)、dpr(24)、gki(12)、mutS(1),recA(1)、thrA(4),是一个新的MLST型,基因型为sly+mrp-ef-。PFGE分析显示NJ25、SH29、SH40与SC84有60%的相似性,RC4726与SC84的相似性仅为20%。用108CFU攻击C57BL/6小鼠,除RC4726外,NJ25、SH29、SH40均能致小鼠发病、死亡。结果表明,NJ25、SH29、SH40可能是高致病性猪链球菌,RC4726是1株非致病性猪链球菌。 |
| 关 键 词： | 猪链球菌 MLST PFGE 病原生物学 |
Pathogenic characteristics of four Streptococcus suis strains |
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| DU Hua-mao,DING Yu-chun,WANG Li-li,CUI Zhi-gang,LIU Pei-hong,YE Chang-yun,XU Jian-guo.Pathogenic characteristics of four Streptococcus suis strains[J].Chinese Journal of Veterinary Science,2010,30(1). | |
| Authors: | DU Hua-mao DING Yu-chun WANG Li-li CUI Zhi-gang LIU Pei-hong YE Chang-yun XU Jian-guo |
| Abstract: | To analyze the pathogenic characteristics of four novel Streptococcus suis isolates,and evaluate their poten-tial threat of sanitation. Screen the virulence-related-genes, pulsed- field gel electrophoresis (PFGE), multiple locus sequence typing (MLST),and experimental animal test. S. suis NJ25 strain belongs to ST7,SH29 and SH40 belong to ST1 ,three strains are sly~+mrp~+ ef~+ genotype. S. suis RC4726 strain seems to be a novel ST,close to ST87,sly~+mrp~-ef~-. The PFGE analysis demonstrated that NJ25, SH29 and SH40 share 60% homology with S. suis SC84 strain which isolated from a patient in Sichuan,China,2005,but RC4726 only 20%. When challenged C57BL/6 mice with a dosage of 10~8 CFU, N J25, SH29, SH40 and SC84 all were lethal with diverse morbidity and mortality, the mice inoculated with RC4726 strain did not fall ill. The latest isolated S. suis strains,NJ25 ,SH29 and SH40 are highly vir-ulence,but RC4726 not. |
| Keywords: | Streptococcus suis MLST PFGE pathogenic biology |
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