| 一株经鸭胚传递的鸭副黏病毒的分离鉴定及生物学特性 |
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| 引用本文： | 纪巍,刁有祥,王明亮,马艳芳,孙宁,孙法良,吴焕荣.一株经鸭胚传递的鸭副黏病毒的分离鉴定及生物学特性[J].中国兽医学报,2010,30(4). |
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| 作者姓名： | 纪巍 刁有祥 王明亮 马艳芳 孙宁 孙法良 吴焕荣 |
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| 作者单位： | 山东农业大学,动物科技学院,山东,泰安,271018 |
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| 摘 要： | 从表现疑似副黏病毒症状的1日龄雏鸭和死亡鸭胚中分离到1株病毒,经HA、HI和病毒中和反应鉴定为副黏病毒,命名为SDFCH株。按常规方法测得SDFCH株的生物学毒力指标MDT、ICPI和IVPI分别为56.6 h、1.78和2.59,鸭胚半数致死量LD50为10-8.5,表明SDFCH株为副黏病毒强毒株。用分离病毒对11日龄鸭胚和7日龄雏鸭攻毒,孵化出的雏鸭和攻毒的雏鸭均出现明显的剖检变化。根据GenBank中NDV F基因序列设计合成了3对引物,通过RT-PCR扩增出了鸭源Ⅰ型禽副黏病毒(APMV-1)SDFCH株的F基因。与已发表副黏病毒F基因相关序列对比结果表明:SDFCH株与鸭、鹅源副黏病毒的同源性较高,核苷酸和氨基酸的同源性均在96.4%~98.0%之间,而与Lasota株同源性较低,核苷酸和氨基酸同源性仅为84.4%和87.9%。表明该毒株相对于传统的Lasota株已经发生了较大的变异。
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| 关 键 词： | 鸭 Ⅰ型禽副黏病毒 F基因 生物学毒力指标 |
Biological identification of a paramyxovirus strain isolated from ducklings and duck-embryos |
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| JI Wei,DIAO You-xiang,WANG Ming-liang,MA Yan-fang,SUN Ning,SUN Fa-liang,WU Huan-rong.Biological identification of a paramyxovirus strain isolated from ducklings and duck-embryos[J].Chinese Journal of Veterinary Science,2010,30(4). |
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| Authors: | JI Wei DIAO You-xiang WANG Ming-liang MA Yan-fang SUN Ning SUN Fa-liang WU Huan-rong |
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| Abstract: | A strain virus isolated from duckling and duck-embryos was identified as paramyxovirus by HA,HI and neutralization of virus.The virus was named SDFCH strain.The MDT,ICPI and IVPI for SDFCH strain were 56.6 h,1.78 and 2.59,respectively.LD_(50) of duck-embryos is 10~(-8.5).The results indicated that SDFCH isolate was viru-lent strain.Ducklings and duck-embryos which were challenged by paramyxovirus SDFCH strain had visible patho-logical change.The fusion (F) gene of SDFCH strain was amplified by the RT-PCR using three pairs of primers de-signed according to the published gene sequences.The F gene of SDFCH strain exhibited 98.4%-98.0% nucleotide and 96.4%-98.0% amino acid homology to those of paramyxoviruses isolated from ducks and gooses.But only 84.4% nucleotide and 87.9% amino acid homology to Lasota strain.The results indicated that SDFCH strain was ge-netically closer to paramyxoviruses isolated from ducks and gooses than to Lasota strain. |
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| Keywords: | duck avian paramyxovirus fusion(F) gene virulence |
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