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| PCR检测临床病例中PCV-2、PRRSV和CSFV的混合感染 | |
| 引用本文： | 何后军,陆承平,罗咏梅.PCR检测临床病例中PCV-2、PRRSV和CSFV的混合感染[J].中国兽医学报,2010,30(2). |
| 作者姓名： | 何后军 陆承平 罗咏梅 |
| 作者单位： | 1. 南京农业大学,动物医学院,江苏,南京,210095;江西农业大学,动物科技学院,江西,南昌,330045 2. 南京农业大学,动物医学院,江苏,南京,210095 3. 江西农业大学,动物科技学院,江西,南昌,330045 |
| 基金项目： | 江西省科技厅重大招标资助项目，江西省教育厅资助项目 |
| 摘 要： | 参照GenBank中已发表的有关基因序列,分别设计合成针对PCV-2、PRRSV和CSFV的3对引物,分别建立检测临床病例中PCV-2、PRRSV和CSFV感染的PCR或RT-PCR方法。结果显示,扩增产物经琼脂糖凝胶电泳,分别各呈现1条大小约1154,372,375bp的特异条带。采用建立的PCR方法对江西各地发病猪和死亡猪的133份临床病料进行PCV-2检测,结果总检出率53.38%(71/133)。在71份PCV-2阳性的病料中检测出PRRSV20份,阳性率28.17%(20/71);CSFV9份,阳性率12.68%(9/71);PRRSV和CSFV共同感染9份,阳性率12.68%(9/71)。 |
| 关 键 词： | 猪圆环病毒2型 猪繁殖与呼吸综合征病毒 猪瘟病毒 混合感染 |
Detection of PCV-2,PRRSV and CSFV mixed infection in clinical cases |
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| HE Hou-jun,LU Cheng-ping,LUO Yong-mei.Detection of PCV-2,PRRSV and CSFV mixed infection in clinical cases[J].Chinese Journal of Veterinary Science,2010,30(2). | |
| Authors: | HE Hou-jun LU Cheng-ping LUO Yong-mei |
| Abstract: | To develop a specific PCR detecting method,three pairs of primers were designed to amplify separately a partial fragment of PCV-2,PRRSV and CSFV, referring to the genome published in Genbank. The PCR or RT-PCR product was separately about 1 154,372 and 375 bp by gelose gel electrophoresis detection. 133 diseased or dead swine specimens collected from different pig farms in Jiangxi were detected by the PCR for PCV-2. It showed 71 specimens were positive for PCV-2, the positive rate was 53.38%. Meanwhile, the 71 specimens were detected by RT-PCR for PRRSV and CSFV, 20 specimens were postive for PRRSV, 9 specimens were postive for CSFV, 9 speci-mens were postive simultaneously for PRRSV and CSFV, the positive rate was separately 28.17%, 12.68% and 12.68%. |
| Keywords: | PCV-2 PRRSV CSFV mixed infection |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |