Seed dormancy mechanisms in warm season grass species |
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| Authors: | Steve W Adkins Sean M Bellairs Don S Loch |
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| Institution: | (1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, P.R. China |
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| Abstract: | Embryogenic protoplasts of Dancy tangerine (Citrus reticulata Blanco) were X-ray irradiated at three doses and electrofused with iodoacetic acid-treated embryogenic protoplasts of Page
tangelo (C. reticulata Blanco × C. paradisi Macf.). Shoots could regenerate only from the fusion combination with the lowest irradiation dose, but were recalcitrant
to rooting. In vitro grafting was applied to obtain complete plants. Chromosome examination showed that the plants contained mainly diploid and
aneuploid cells, together with few tetraploid cells, indicating that they were mixoploids. Random amplified polymorphic DNA
analyses with 10-mer arbitrary primers confirmed the plants as true somatic hybrids. This is the first report on regeneration
of mixoploid hybrid plants via protoplast asymmetric fusion in Citrus. Negative effects of ionizing irradiation on regeneration of embryoids and plantlets and possible agronomic interest of the
mixoploid plants are also discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | aneuploid asymmetric fusion Citrus mixoploid hybrid RAPD X-ray |
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