| 大蒜鳞茎中抗番茄灰霉病内生菌的筛选及其防治效果 |
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| 引用本文： | 邓振山,马娜娜,徐文梅.大蒜鳞茎中抗番茄灰霉病内生菌的筛选及其防治效果[J].西北农林科技大学学报(社会科学版),2012,40(5):50-58. |
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| 作者姓名： | 邓振山 马娜娜 徐文梅 |
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| 作者单位： | 延安大学 生命科学学院;延安大学 生命科学学院;延安大学 生命科学学院 |
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| 基金项目： | 陕西省科技厅重大创新项目(2009ZKC-04-09) |
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| 摘 要： | 【目的】筛选拮抗番茄灰霉病的内生菌菌株，研究其生防效果，为开发有应用潜力的微生物农药奠定基础。【方法】用平板对峙法从大蒜鳞茎内筛选具有拮抗作用的内生菌，测定拮抗菌株对番茄离体叶片、果实、胚根和盆栽植株灰霉病的防治效果，以及对7种常见病原真菌的抑菌活性。【结果】从分离纯化的63株候选菌株中获得了22株具有一定抑菌活性的内生菌，其中有4株内生菌(D6,D10,D21,Y3)对番茄灰霉病有显著的抑制效果，抑菌率最高可达77.5%。4株拮抗菌均有较强的抑菌活性，对番茄离体叶片、果实和胚根上番茄灰霉病菌的生长抑制率最高分别达92%，71.13%和70.3%；对盆栽植株番茄灰霉病菌的抑制率最高可达96.2%；这4株菌对西瓜枯萎病菌(Fusarium oxysporum f.sp.niveum)、玉米弯孢病菌(Curvularia lunata)、烟草赤星病菌(Alternaria longipes)、小麦赤霉病菌(Fusarium graminearum)、苹果炭疽病菌(Glomerella cingulata)、稻瘟病菌(Pyricularia oryzae Cav.)和棉花枯萎病菌(Fusarium oxysporun f.sp.vasinfectum)均有一定的抑菌活性，抑制率最高达83.1%。【结论】大蒜鳞茎中的内生菌对番茄灰霉病有明显的抑制作用，这为内生菌在番茄灰霉病防治方面的应用提供了理论依据。
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| 关 键 词： | 大蒜鳞茎 内生菌 番茄灰霉病 防治效果 |
| 收稿时间： | 2011/11/7 0:00:00 |
Screening endophytic bacteria strains of anti Botrytis cinerea from the garlic bulb and their control effect |
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| DENG Zhen-shan,MA Na-na,XU Wen-mei,LI Jun,WEI Ge-hong.Screening endophytic bacteria strains of anti Botrytis cinerea from the garlic bulb and their control effect[J].Journal of Northwest Sci-Tech Univ of Agr and,2012,40(5):50-58. |
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| Authors: | DENG Zhen-shan MA Na-na XU Wen-mei LI Jun WEI Ge-hong |
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| Institution: | 1 The Life-science Department,Yan’an University,Yan’an,Shaanxi 716000,China;2 Yan’an Institute of Microbiology,Yan’an, Shaanxi 716000,China;3 College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | 【Objective】 The experiment was conducted to isolate endophytic bacteria strains of antagonistic to Botrytis cinerea,to investigate the control effect,and to explore the non-pollution control methods.【Method】 Endophytic bacteria were isolated from the garlic bulb with the antagonism experiment by face-to-face culturing on PDA media method.Under condition in vitro of tomato leaves,fruits,radicles and potted plants,their prevention and control effect to B.cinerea were measured.【Result】 The results showed that four strains of them(D6,D10,D21,Y3) had a striking inhibitory effect,the highest inhibitory rate was up to 77.5%;The antibiotic effects of tomato in vitro test showed that four antagonistic strains had a strong antagonistic activity against B.cinerea.The highest inhibitory rate of leaves,the fruit and radicle in vitro reached 92%,71.13% and 70.3%,respectively.The pot experiment determination of inhibitory rate was up to 96.2%.The antimicrobial spectrum test showed that four strains expressed antibiotic activity on Fusarium oxysporum f.sp.niveum,Curvularia lunata,Alternaria longipes,Fusarium graminearum,Glomerella cingulata,Pyricularia oryzae Cav.,Fusarium oxysporun f.sp.vasinfectum,the highest inhibition rate was up to 83.1%.【Conclusion】 Endophytic bacteria isolated from the garlic bulb have a striking inhibitory effect to B.cinerea.The research provides a theoretical basis for utilizing microbial control of endophytic bacteria in B.cinerea. |
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| Keywords: | garlic bulb endophytic bacteria Botrytis cinerea control effects |
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