| 甘蔗遗传转化中膦丝菌素(PPT)筛选效率的比较 |
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| 引用本文： | 王继华,张木清,曹干.甘蔗遗传转化中膦丝菌素(PPT)筛选效率的比较[J].中国糖料,2011(3). |
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| 作者姓名： | 王继华 张木清 曹干 |
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| 作者单位： | 福建农林大学生命科学学院;广东省农业科学院作物研究所; |
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| 基金项目： | 国家“863”项目:斑茅FKBP12基因在甘蔗抗旱上的作用网络研究(2008AA10Z114); 广东省自然科学基金:转Bt基因甘蔗的外源基因表达分析及其与抗虫性关系(10151064001000032); 广东省科技攻关项目:抗旱转基因甘蔗选育(2010B020301001) |
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| 摘 要： | 从PCR检测水平评价了PPT在再生过程,再生植株喷施和体外浸泡中筛选方法的效率,结果表明PPT浸泡方式效率最高,能够准确鉴定出转Bar基因甘蔗。
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| 关 键 词： | 甘蔗 筛选效率 转化 |
Screening Efficiency of Phosphinothricin(PPT) in Sugarcane Transformation |
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| WANG Ji-hua,ZHANG Mu-qing,CAO Gan.Screening Efficiency of Phosphinothricin(PPT) in Sugarcane Transformation[J].Sugar Crops of China,2011(3). |
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| Authors: | WANG Ji-hua ZHANG Mu-qing CAO Gan |
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| Institution: | WANG Ji-hua1,2,ZHANG Mu-qing1,CAO Gan2(1.College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 50002,China,2.Crop Research Institute,Guangdong Academy of Agriculture Sciences,Guangzhou 510640,China) |
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| Abstract: | Bar gene as a common marker is widely used in transgenic sugarcane.The research was conducted to evaluate the screening efficiency of PPT used at the stage of regeneration and the regeneration plants by spraying or in vitro soaking.The results showed that the highest screening efficiency was found in the regenerated plants by soaking in vitro which could accurately identify the transgenic sugarcane mediated with Bar gene. |
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| Keywords: | sugarcane screening efficiency transformation |
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